Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Luciano A. Masullo"'
Autor:
Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Mauricio Pilo-Pais, Guillermo P. Acuna, Fernando D. Stefani
Publikováno v:
Light: Science & Applications, Vol 11, Iss 1, Pp 1-9 (2022)
Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale. Recently, the method called MINFLUX has achieved a ~10-fold improvement in spatial resolution over previously developed techniques,
Externí odkaz:
https://doaj.org/article/59e88291081343f3af19747b9c77544a
Publikováno v:
Light: Science & Applications, Vol 11, Iss 1, Pp 1-4 (2022)
Abstract Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with t
Externí odkaz:
https://doaj.org/article/3068cf661ec04ec9a3fa144c554e5a04
Publikováno v:
Biophysical Reports, Vol 2, Iss 1, Pp 100036- (2022)
Localization of single fluorescent molecules is key for physicochemical and biophysical measurements, such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Over the last two decades, several methods
Externí odkaz:
https://doaj.org/article/ef86ad55fa6e459b801b079d7549ac9a
Autor:
Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Mauricio Pilo-Pais, Guillermo P. Acuna, Fernando D. Stefani
Publikováno v:
Light: Science & Applications, Vol 11, Iss 1, Pp 1-1 (2022)
Externí odkaz:
https://doaj.org/article/c7a729af8d554a6fa313f61c5aa7a3e3
Autor:
Luciano A. Masullo, Andreas Bodén, Francesca Pennacchietti, Giovanna Coceano, Michael Ratz, Ilaria Testa
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Super-resolution microscopy often suffers from low contrast and slow recording times. Here the authors present an optical implementation which makes the fluorescent proteins’ ON–OFF switching cycles more efficient, enhancing contrast and spatio-t
Externí odkaz:
https://doaj.org/article/7cc42c8ba8c447d2a6cf7482a6916e8d
Autor:
Susanne C. M. Reinhardt, Luciano A. Masullo, Isabelle Baudrexel, Philipp R. Steen, Rafal Kowalewski, Alexandra S. Eklund, Sebastian Strauss, Eduard M. Unterauer, Thomas Schlichthaerle, Maximilian T. Strauss, Christian Klein, Ralf Jungmann
Publikováno v:
Nature. 617:711-716
Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in the life sciences to understand complex biological systems. Super-resolution approaches1–6 can achieve resolution in cells in the range of
Autor:
Eduard M. Unterauer, Sayedali Shetab Boushehri, Kristina Jevdokimenko, Luciano A. Masullo, Mahipal Ganji, Shama Sograte-Idrissi, Rafal Kowalewski, Sebastian Strauss, Susanne C.M. Reinhardt, Ana Perovic, Carsten Marr, Felipe Opazo, Eugenio F. Fornasiero, Ralf Jungmann
SUMMARYTo fully understand biological processes and functions, it is necessary to reveal the molecular heterogeneity of cells and even subcellular assemblies by gaining access to the location and interaction of all biomolecules. The study of protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07408db5a2c800b9008d7361e669edcf
https://doi.org/10.1101/2023.05.17.541210
https://doi.org/10.1101/2023.05.17.541210
Autor:
Lucía F. Lopez, Luciano A. Masullo, Alan M. Szalai, Florencia Edorna, Florencia D. Choque, Fernando Caprile, Fernando D. Stefani
Publikováno v:
Journal of the Optical Society of America B. 40:C103
Single-molecule localization microscopy (SMLM) has become an essential tool to investigate phenomena at the nanoscale. Among the different SMLM approaches, methods that interrogate the molecular position with an intensity minimum, such as minimal emi
Focused optical fields are key to a multitude of applications involving light-matter interactions, such as optical microscopy, single-molecule spectroscopy, optical tweezers, lithography, or quantum coherent control. A detailed vectorial characteriza
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::280f1d9564344845566de4474489968e
http://arxiv.org/abs/2110.00160
http://arxiv.org/abs/2110.00160
Publikováno v:
Biophys Rev
Fluorescence nanoscopy represented a breakthrough for the life sciences as it delivers 20–30 nm resolution using far-field fluorescence microscopes. This resolution limit is not fundamental but imposed by the limited photostability of fluorophores