Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Luc-Alain Savoy"'
Autor:
Luc-Alain Savoy, Jean-Claude Jaton, Andrew J. Reason, Séverine Frutiger-Hughes, Graham J. Hughes
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1434:86-93
Human secretory component has seven putative sites for N -linked glycosylation. From tryptic and Glu-C digests we have isolated peptides encompassing asparagines 65, 72, 117, 168, 403, 451 and 481. Analysis by on line HPLC-electrospray mass spectrome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf487543e1c6e040de102ba9bfb6439a
https://doi.org/10.1016/s0167-4838(99)00168-5
https://doi.org/10.1016/s0167-4838(99)00168-5
Autor:
Andreas Tinschert, M. Olwen Birch, Veronika Venetz, M. Nicholas Shaw, Luc-Alain Savoy, Rüdiger Dietrich
Publikováno v:
Biochemical Journal. 330:1079-1085
A cell-free extract from Escherichia coli containing an E. coli biotin synthase that was expressed to approx. 1% of soluble cell protein by cloning the E. coli bioB gene was used to investigate the biotin synthase reaction. The pH optimum was between
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c978bf3a851b02df110f02ab96fca129
https://doi.org/10.1042/bj3301079
https://doi.org/10.1042/bj3301079
Publikováno v:
European Journal of Biochemistry. 222:761-767
Advanced mass spectrometric procedures have been extensively used to provide an accurate structural characterization of aspartate aminotransferase from Sulfolobus solfataricus. The amino acid sequence of this enzyme had previously been deduced from t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b8798453a24d3b0669beca1f352a3a
https://doi.org/10.1111/j.1432-1033.1994.tb18922.x
https://doi.org/10.1111/j.1432-1033.1994.tb18922.x
Autor:
G M Clore, P O Regamey, Angela M. Gronenborn, Paul T. Wingfield, M G Simona, B N Green, Luc-Alain Savoy, Keith Rose
Publikováno v:
Journal of Biological Chemistry. 267:19101-19106
Ner protein of bacteriophage Mu, produced by recombinant DNA techniques in Escherichia coli, has been found to possess a molecule of pyruvic acid attached covalently through carbon-2 to the amino-terminal cysteine residue. The intact protein and the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::43b130e3b381dcfa14d75a7adc2d0a66
https://doi.org/10.1016/s0021-9258(18)41747-4
https://doi.org/10.1016/s0021-9258(18)41747-4
Publikováno v:
Peptides 1992 ISBN: 9789401046466
Peptides 1992
Peptides 1992
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e93c94d725303c9d63856ad4f5b1e442
https://doi.org/10.1007/978-94-011-1470-7_193
https://doi.org/10.1007/978-94-011-1470-7_193
Autor:
Reto Stöcklin, Pierre Vuagnat, Robin E. Offord, Pierre-Olivier Regamey, Luc-Alain Savoy, Jan Markussen, Keith Rose
Publikováno v:
Protein engineering. 4(4)
The production of semisynthetic human insulin for therapeutic purposes is of considerable importance. During trypsin-catalysed transformation of pig insulin into an ester of insulin of human sequence, the alanyl residue at position B30 is removed and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b74ffaaca697fc334eed8bd939a50817
https://pubmed.ncbi.nlm.nih.gov/1881866
https://pubmed.ncbi.nlm.nih.gov/1881866
Publikováno v:
Biochemical Journal. 250:253-259
A previously described technique [Rose, Simona, Offord, Prior, Otto & Thatcher (1983) Biochem. J. 215, 273-277] permits the identification of the C-terminal peptide of a protein as the only peptide that does not incorporate any 18O upon partial enzym
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90cc03d0bf58feb94997deeb795f27ca
https://doi.org/10.1042/bj2500253
https://doi.org/10.1042/bj2500253
Autor:
Robin Ewart Offord, Luc-Alain Savoy, David R. Thatcher, Keith Rose, Paul T. Wingfield, M G Simona, R J Mattaliano
Publikováno v:
Analytical Biochemistry. 165:59-69
The removal of N-terminal methionine from proteins produced by recombinant DNA techniques is often far from quantitative. Furthermore, a proportion of the methionylated product may be N α -blocked and thus not easily accessible to conventional (Edma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c0dc99ec9b1074b40b3c03270036161
https://doi.org/10.1016/0003-2697(87)90201-6
https://doi.org/10.1016/0003-2697(87)90201-6
Autor:
Andrew J. Reason, Graham J. Hughes, Luc-Alain Savoy, Howard R. Morris, Jean-Claude Jaton, Séverine Frutiger
Publikováno v:
FEBS Letters. (2-3):443-446
The main objective of this work was to unequivocally determine the C-terminal sequence of human milk free secretory component (SC). It was found to end at arginine-585, i.e. 33 amino acids downstream from the major heterogeneous C-terminal residue pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::257300910d9c00f34e985a493ebe8bd4
Autor:
Károly Kovács, Luc-Alain Savoy, John Halstrøm, Pierre Vuagnat, Keith Rose, Klavs H. Jørgensen
Publikováno v:
"Protein Engineering, Design and Selection". 2:313-315
The title compound was prepared by catalytic deiodination of (Tyr-A14-3-I) insulin with deuterium gas. Under special conditions (large excess of PdO in CH3OD/D2O at pH 9.2) developed to minimize problems due to poisoning of the catalyst, deuterated i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6faef9571873e81bc666a33249a9ffeb
https://doi.org/10.1093/protein/2.4.313
https://doi.org/10.1093/protein/2.4.313