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pro vyhledávání: '"Lubov Sakovina"'
Publikováno v:
International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 13460
The genome editing approach using the components of the CRISPR/Cas system has found wide application in molecular biology, fundamental medicine and genetic engineering. A promising method is to increase the efficacy and specificity of CRISPR/Cas-base
Autor:
Daria Kim, D. S. Novopashina, Lubov Sakovina, Ivan Vokhtantsev, Elizaveta Akhmetova, Victor M. Golyshev, Mariya A. Vorobyeva, Sergey Novopashin, Olga A. Semikolenova
Publikováno v:
International Journal of Molecular Sciences
Volume 22
Issue 20
International Journal of Molecular Sciences, Vol 22, Iss 10919, p 10919 (2021)
Volume 22
Issue 20
International Journal of Molecular Sciences, Vol 22, Iss 10919, p 10919 (2021)
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA. PC-DNAs c