Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Luben B. Dolapchiev"'
Autor:
Marina N. Nedelcheva, Assen Roguev, Hristo B. Taskov, Luben B. Dolapchiev, A. Francis Stewart, Andrej Shevchenko, Anna Shevchenko, Stoyno S. Stoynov
Publikováno v:
Journal of Molecular Biology. 347:509-521
The replicative DNA helicases can unwind DNA in the absence of polymerase activity in vitro. In contrast, replicative unwinding is coupled with DNA synthesis in vivo. The temperature-sensitive yeast polymerase alpha/primase mutants cdc17-1, pri2-1 an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c8da766a47ef6db5fb7fa845f44d32
https://doi.org/10.1016/j.jmb.2005.01.041
https://doi.org/10.1016/j.jmb.2005.01.041
Publikováno v:
Biotechnology and Bioengineering. 51:488-493
Studies were conducted on Nicotiana tabacum 1507 cultivation in an aqueous two-phase system (PD(5)) formed by adding 4% PEG (MW 20,000) and 7.5% dextran (MW 70,000) to the medium. The time course of growth and changes in the phase volumes of the PD(5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3990abdcd2d0b9fcd983593a76703865
https://doi.org/10.1002/(sici)1097-0290(19960820)51:4<488::aid-bit12>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19960820)51:4<488::aid-bit12>3.0.co
Publikováno v:
The International Journal of Biochemistry & Cell Biology. 31:1399-1407
The 5′→3′ exonuclease from beef spleen is a 160-kDa tetramer consisting of four subunits of two types. Partial reduction of the tetramer led to one stable intermediate state of the enzyme with M r =80 kDa. The aim of this paper was to attribute
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4065a29a71966df66cbc6db3425204a7
https://doi.org/10.1016/s1357-2725(99)00108-9
https://doi.org/10.1016/s1357-2725(99)00108-9
Autor:
Luben B. Dolapchiev, Svetoslav I. Dimov, Atanaska V. Mitkova, Anastassia T. Bakalova, Stoyno S. Stoynov
Publikováno v:
FEBS Letters. 409:151-154
Polyclonal antibodies against the exonuclease from Crotalus adamanteus venom (the 140-kDa protein) inhibit both the exonucleolytic and the single-strand-specific endonucleolytic activities, present in the exonuclease preparation. The antibodies also
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6977ef7a8bbd5913c7a0373bc18333a
https://doi.org/10.1016/s0014-5793(97)00489-4
https://doi.org/10.1016/s0014-5793(97)00489-4
Autor:
Mladenka Ilieva, A. Bakalova, Luben B. Dolapchiev, Magdalina D. Mihneva, Rossitsa A. Vassileva
Publikováno v:
Enzyme and Microbial Technology. 19:384-388
A new, fast, and easy assay based on the “cell squash” method for analysis of a large number of tobacco cell cultures which produces phosphohydrolytic enzymes is described. The time course of growth and changes in the amount of the secreted phosp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf60dfa625f133c58dc1d63a67d07397
https://doi.org/10.1016/s0141-0229(96)00025-7
https://doi.org/10.1016/s0141-0229(96)00025-7
Autor:
Luben B. Dolapchiev, Stoyno S. Stoynov
Publikováno v:
The international journal of biochemistrycell biology. 33(2)
All studied origins of replication of DNA in Saccharomyces cerevisiae contain DNA unwinding elements. The introduction of unrestrained negative supercoiling leads to melting of the two DNA strands in DNA unwinding elements. To understand the mechanis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1d79d3e98f6ea3083ee785c31f69f2f
https://pubmed.ncbi.nlm.nih.gov/11240374
https://pubmed.ncbi.nlm.nih.gov/11240374
Publikováno v:
Archives of biochemistry and biophysics. 351(2)
A one-step purification of beef spleen exonuclease in the form of a DNA–enzyme complex is described. The purity of the exonuclease was verified by two-dimensional gel electrophoresis. It possesses molecular mass 160 kDa and p I 6.92. The one-dimens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1be7674702da5e5682fa888fc34fd880
https://pubmed.ncbi.nlm.nih.gov/9515059
https://pubmed.ncbi.nlm.nih.gov/9515059
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 622:331-336
Exonuclease from Crotalus adamanteus venom has only threonine as N-terminimal amono acid residue. It was examined for its amino acid composition, -SH and S-S groups. It has no free -SH groups and seven S-S bonds. The analysis of the carbohydrate resi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a20acc7558c6bc9b446d809acf699bdc
https://doi.org/10.1016/0005-2795(80)90044-6
https://doi.org/10.1016/0005-2795(80)90044-6
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 667:355-360
The immunoelectrophoretic purity of the exonuclease preparation, isolated from Crotalus adamanteus venom according to a procedure previously published (Dolapchiev, L.B., Sulkowski, E. and Laskowski, M., Sr. (1974) Biochem. Biophys. Res. Commun. 61, 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::836552fc5d51c201292f45b765b671e1
https://doi.org/10.1016/0005-2795(81)90202-6
https://doi.org/10.1016/0005-2795(81)90202-6
Publikováno v:
Journal of Biochemical and Biophysical Methods. 17:207-213
Some of the synthetic chromophoric substrates of various enzymes cannot be used for direct spectrophotometric recording of the reactions, when a difference between the pH optimum of the enzyme reaction and the pH of maximum absorption of the released
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0225bc29151ba881a16b5cc7b7a691fd
https://doi.org/10.1016/0165-022x(88)90006-1
https://doi.org/10.1016/0165-022x(88)90006-1