[Effect of Bacillus subtilis A-50 'streptomycin' mutations on the formation of molecular forms of subtilisin, an extracellular alkaline proteinase]

Autor: Lm, Ermakova, Zt, Abramov, Alex Strongin, Ls, Izotova, Ev, Zakharova

Publikováno v: Europe PubMed Central

The patterns of subtilisin molecular forms of streptomycin-resistant (Strr) and streptomycin-dependent (Strd) mutants of Bacillus subtilis A-50, as well as the revertants of Strd to streptomycin-independence (Str1) were studied. Strr mutants had diff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7c21d63129b28ce70725c2b76b3301d9
https://pubmed.ncbi.nlm.nih.gov/102561

[Metalloproteinase from Bacillus subtilis: - 'intracellular' and extracellular enzymes]

Autor: Ka, Shaginian, Ls, Izotova, Iomantac IuV, Alex Strongin, Vm, Stepanov

Publikováno v: Europe PubMed Central

"Intracellular" metalloproteinase was purified to homogeneity from Bacillus subtilis 103 crude cell extract, using affinity chromatography on bacitracin-Sepharose 4B. The degree of purification and the yield of the enzyme were about 260-fold and 3%,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::951889ca8609991b4d703601c6759886
http://europepmc.org/abstract/med/6786372

[Isolation of proteinase I from E. coli cells using affinty chromatography on bacitracin-Sepharose]

Autor: Ka, Shaginian, Ls, Izotova, Alex Strongin, Vm, Stepanov

Publikováno v: Europe PubMed Central

A serine proteinase (proteinase I) was isolated in a homogeneous state from E. coli K12 cells, using bacitracin-Sepharose 4B affinity chromatography. The enzyme effectively cleaved N alpha-acetyl-L-phenylalanine beta-naphthyl ester. The proteinase wa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::e4c30bd4a9f3b6c106c180eab580b8f3
http://europepmc.org/abstract/med/6991002

Ligand-independent interaction of the type I interferon receptor complex is necessary to observe its biological activity.

Autor: Krause CD; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, The University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, NJ 08855, USA. Electronic address: christopher.d.krause@gmail.com., Digioia G, Izotova LS, Xie J, Kim Y, Schwartz BJ, Mirochnitchenko OV, Pestka S

Publikováno v: Cytokine [Cytokine] 2013 Oct; Vol. 64 (1), pp. 286-97. Date of Electronic Publication: 2013 Jul 03.

Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmid.

Autor: Krause CD; Department of Molecular Genetics, Microbiology, and Immunology, RWJMS-UMDNJ, 675 Hoes Lane West, Piscataway, NJ 08854, USA., Izotova LS, Ren G, Yuan ZR, Shi Y, Chen CC, Ron Y, Pestka S

Publikováno v: Stem cell research & therapy [Stem Cell Res Ther] 2011 Mar 14; Vol. 2 (2), pp. 15. Date of Electronic Publication: 2011 Mar 14.