In-solution buffer-free digestion for the analysis of SARS-CoV-2 RBD proteins allows a full sequence coverage and detection of post-translational modifications in a single ESI-MS spectrum

Autor: Gleysin Cabrera, D. Gonzalez, Lozada-Chang Sl, Tammy Boggiano, de la Luz Hernández Kr, Glay Chinea, González I, Luis Javier González, Palacio J, Vérez-Bencomo, Hernández L, Gertrudis Rojas, Gerardo Guillén, Becquet M, Marika, Besada, Luis Ariel Espinosa, Pérez-Massón B, Andújar I, Santana-Medero D, Yury Valdés-Balbín, Marta Ayala, Pimentel E, Limonta M, Camila Canaán-Haden, Daniel G. Rivera, Yassel Ramos, Mark Emalfarb, Torres Eo, Saloheimo Markku, Alejandro Martín, Ronen Tchelet, Pérez-Martínez D, Nelson E

Subunit vaccines based on the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2, are among the most promising strategies to fight the COVID-19 pandemic. The detailed characterization of the protein primary structure by mass spectrometr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9c9ea09aebf5a17d879eeb702ba9f449
https://doi.org/10.1101/2021.05.10.443404

Conformational characterization of the mammalian-expressed SARS-CoV-2 recombinant receptor binding domain, a COVID-19 vaccine.

Autor: Moro-Pérez L; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba. leina@cim.sld.cu., Boggiano-Ayo T; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba. tamy@cim.sld.cu., Lozada-Chang SL; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba., Fernández-Saiz OL; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba., de la Luz KR; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba., Gómez-Pérez JA; Bioprocess R&D Department, Center of Molecular Immunology, 216 Street and 15 Avenue, Atabey, Playa, P.O. Box 16040, 11600, Havana, Cuba.

Publikováno v: Biological research [Biol Res] 2023 May 08; Vol. 56 (1), pp. 22. Date of Electronic Publication: 2023 May 08.

In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI-MS spectrum.

Autor: Espinosa LA; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Ramos Y; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Andújar I; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Torres EO; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Cabrera G; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Martín A; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Roche D; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Chinea G; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Becquet M; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., González I; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Canaán-Haden C; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Nelson E; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Rojas G; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Pérez-Massón B; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Pérez-Martínez D; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Boggiano T; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Palacio J; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Lozada Chang SL; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Hernández L; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., de la Luz Hernández KR; Center of Molecular Immunology, 216 St., P.O. Box 16040, Havana, Cuba., Markku S; VTT Technical Research Centre of Finland Ltd, P.O. Box 1000, 02044 VTT, Espoo, Finland., Vitikainen M; VTT Technical Research Centre of Finland Ltd, P.O. Box 1000, 02044 VTT, Espoo, Finland., Valdés-Balbín Y; Finlay Vaccine Institute, 200 and 21 Street, 11600, Havana, Cuba., Santana-Medero D; Finlay Vaccine Institute, 200 and 21 Street, 11600, Havana, Cuba., Rivera DG; Laboratory of Synthetic and Biomolecular Chemistry, Faculty of Chemistry, University of Havana, Zapata & G, 10400, Havana, Cuba., Vérez-Bencomo V; Finlay Vaccine Institute, 200 and 21 Street, 11600, Havana, Cuba., Emalfarb M; Dyadic International, Inc, 140 Intercoastal Pointe Drive, Suite #404, Jupiter, FL, 33477, USA., Tchelet R; Dyadic International, Inc, 140 Intercoastal Pointe Drive, Suite #404, Jupiter, FL, 33477, USA., Guillén G; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Limonta M; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Pimentel E; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Ayala M; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., Besada V; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba., González LJ; Center for Genetic Engineering and Biotechnology, Ave 31, e/ 158 y 190, Cubanacán, Playa, Havana, Cuba. luis.javier@cigb.edu.cu.

Publikováno v: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Dec; Vol. 413 (30), pp. 7559-7585. Date of Electronic Publication: 2021 Nov 05.