Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Louisette J. Basa"'
Autor:
Alavattam Sreedhara, Zephania W. Kwong Glover, Jennifer S. Laurence, Benjamin Moore, Louisette J. Basa
Publikováno v:
mAbs. 7:901-911
Fragmentation in the hinge region of an IgG1 monoclonal antibody (mAb) can affect product stability, potentially causing changes in potency and efficacy. Metals ions, such as Cu(2+), can bind to the mAb and undergo hydrolysis or oxidation, which can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8aa6d5aca860c13dd9adf231304ef967
https://doi.org/10.1080/19420862.2015.1062193
https://doi.org/10.1080/19420862.2015.1062193
Autor:
Louisette J. Basa
Publikováno v:
Methods in Molecular Biology ISBN: 9781627035408
This chapter describes an LC-ESI-MS method for the DAR and drug load distribution analysis that is suitable for lysine-linked ADCs. The ADC sample is desalted using a reversed-phase LC column with an acetonitrile gradient prior to online MS analysis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bfe76eee867d3b27f14a0ee76aedbf1
https://doi.org/10.1007/978-1-62703-541-5_18
https://doi.org/10.1007/978-1-62703-541-5_18
Autor:
Michael W. Spellman, Victor Ling, Reed J. Harris, Kenneth J. Smith, Louisette J. Basa, John Glushka, Herman van Halbeek
Publikováno v:
Biochemistry. 32:6539-6547
O-Linked fucose has been found attached to Thr/Ser residues within the sequence Cys-X-X-Gly-Gly-Thr/Ser-Cys in the N-terminal EGF domains of several coagulation/fibrinolytic proteins. Carbohydrate composition and mass spectrometric analyses of trypti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98ba84ff212d1fdef966c20c627b2e2e
https://doi.org/10.1021/bi00077a007
https://doi.org/10.1021/bi00077a007
Autor:
Avi Ashkenazi, Richard F. Weber, Diane Pennica, David V. Goeddel, Michael W. Spellman, Van T. Lam, Louisette J. Basa, Steven J. Shire, William J. Kohr
Publikováno v:
Biochemistry. 32:3131-3138
An expression plasmid encoding the extracellular domain of the 75-kDa human tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable cell line secreting soluble TNF-R2 (sTNF-R2). Purified sTNF-R2 was resolved
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22a802f9a5b6cbda6434fea54de0c9a0
https://doi.org/10.1021/bi00063a027
https://doi.org/10.1021/bi00063a027
Publisher Summary This chapter describes the application of high-pH anion-exchange chromatography, with pulsed amperometric detection (HPAEC-PAD) to the identification and partial characterization of glycopeptides, within a reversed-phase high-perfor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::928cd9344fe56c346e5c4475f1e5f8ac
https://doi.org/10.1016/s0076-6879(96)71008-x
https://doi.org/10.1016/s0076-6879(96)71008-x
Autor:
Jia-Yan Wu, Michael W. Spellman, Amy Lim, Jeffrey L. Cleland, Michael F. Powell, Deborah A. Wheeler, Louisette J. Basa, Charlotte R. Kensil, Neil E. Jacobsen
Publikováno v:
Journal of pharmaceutical sciences. 85(1)
The stability of the immunologic adjuvant QS-21 (Cambridge Biotech Corp.) was optimized for use in the MN rgp120 HIV-1 subunit vaccine. QS-21, a saponin purified by reversed phase HPLC from an extract of the bark of the Quillaja saponaria Molina tree
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc41c9b5cb4ba6e0ab8cc45f261d8282
https://pubmed.ncbi.nlm.nih.gov/8926578
https://pubmed.ncbi.nlm.nih.gov/8926578
Autor:
Michael W. Spellman, Russell W. Carlson, Kenneth J. Smith, Reed J. Harris, John Glushka, Victor Ling, Louisette J. Basa, Herman van Halbeek
Publisher Summary This chapter explores the structural characterization of a novel tetrasaccharide attached to ser-61 of human factor ix by mass spectrometry and 1H NMR spectroscopy. In a study described in the chapter, trypsin-digested S-carboxymeth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::75b82c7be6c4810f78c55376a350d052
https://doi.org/10.1016/b978-0-12-194710-1.50013-8
https://doi.org/10.1016/b978-0-12-194710-1.50013-8
Publikováno v:
Analytical chemistry. 65(21)
Electrospray ionization mass spectrometry utilizing a single quadrupole on line with reversed-phase HPLC (LC/MS) enables the characterization of glycoproteins in a relatively short period of time. In this approach the protein is digested with a suita
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da35a1a44b6b5a144b1b9c2ef458e225
https://pubmed.ncbi.nlm.nih.gov/8256861
https://pubmed.ncbi.nlm.nih.gov/8256861
Publikováno v:
Biochemistry. 30(9)
Infection of T-lymphocytes and macrophages by human immunodeficiency virus (HIV) is mediated by the binding of the HIV envelope glycoprotein to the cell-surface receptor glycoprotein CD4. A soluble, recombinant CD4 molecule (rCD4), produced by expres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::473b4d4aed64cbe36f934bdee9206663
https://pubmed.ncbi.nlm.nih.gov/2001369
https://pubmed.ncbi.nlm.nih.gov/2001369
Autor:
Michael W. Spellman, Louisette J. Basa
Publikováno v:
Journal of chromatography. 499
The technique of high-pH anion-exchange chromatography with pulsed amperometric detection has recently been shown to be a powerful method for resolving closely related oligosaccharides [M. R. Hardy and R. R. Townsend, Proc. Natl. Acad. Sci. U.S.A., 8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6f0e9fa16af07727a166c07bbb88c65
https://pubmed.ncbi.nlm.nih.gov/2324208
https://pubmed.ncbi.nlm.nih.gov/2324208