Modeling human skeletal development using human pluripotent stem cells

Autor: Shireen R. Lamandé, Elizabeth S. Ng, Trevor L. Cameron, Louise H. W. Kung, Lisa Sampurno, Lynn Rowley, Jinia Lilianty, Yudha Nur Patria, Tayla Stenta, Eric Hanssen, Katrina M. Bell, Ritika Saxena, Kathryn S. Stok, Edouard G. Stanley, Andrew G. Elefanty, John F. Bateman

Publikováno v: Proceedings of the National Academy of Sciences. 120

Chondrocytes and osteoblasts differentiated from induced pluripotent stem cells (iPSCs) will provide insights into skeletal development and genetic skeletal disorders and will generate cells for regenerative medicine applications. Here, we describe a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f44005e5885c5223c651c0ebe78aa93d
https://doi.org/10.1073/pnas.2211510120

Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing

Autor: Lisa Sampurno, John F. Bateman, Shireen R. Lamandé, Louise H. W. Kung, Christopher B. Little

Publikováno v: Stem Cell Research, Vol 50, Iss, Pp 102118-(2021)

miR-26b has been implicated in a wide range of human diseases, including cancer, diabetes, heart disease, Alzheimer’s disease and osteoarthritis. To provide a tool to explore the importance of miR-26b in this broad context, we have generated and ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5154df176feb6cb4b02eabcf89f7367d
https://pubmed.ncbi.nlm.nih.gov/33316599

Comprehensive Expression Analysis of microRNAs and mRNAs in Synovial Tissue from a Mouse Model of Early Post-Traumatic Osteoarthritis

Autor: John F. Bateman, Varshini Ravi, Louise H. W. Kung, Lynn Rowley, Katrina M. Bell, Christopher B. Little

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
Scientific Reports

To better understand the molecular processes involved in driving osteoarthritis disease progression we characterized expression profiles of microRNAs (miRNA) and mRNAs in synovial tissue from a post-traumatic OA mouse model. OA was induced in 10–12

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88ad48ac126222b064d9d09e0e584b7b
http://link.springer.com/article/10.1038/s41598-017-17545-1

Cartilage endoplasmic reticulum stress may influence the onset but not the progression of experimental osteoarthritis

Autor: Louise H. W. Kung, Lorna Mullan, Jamie Soul, Ping Wang, Kazutoshi Mori, John F. Bateman, Michael D. Briggs, Raymond P. Boot-Handford

Publikováno v: Kung, L, Mullan, L, Soul, J, Wang, P, Mori, K, Bateman, J F, Briggs, M D & Boot-Handford, R 2019, ' Cartilage endoplasmic reticulum stress may influence the onset but not the progression of experimental osteoarthritis ', Arthritis Research & Therapy . https://doi.org/10.1186/s13075-019-1988-6
Arthritis Research & Therapy, Vol 21, Iss 1, Pp 1-13 (2019)

Background: Osteoarthritis has been associated with a plethora of pathological factors and one which has recently emerged is chondrocyte endoplasmic reticulum (ER) stress. ER stress is sensed by key ER-resident stress-sensors, one of which is activat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::c90f622ed477eb23518eb7672bc97884
https://doi.org/10.1186/s13075-019-1988-6

Cartilage microRNA dysregulation in mouse osteoarthritis overlaps with patient disease candidates

Autor: John F. Bateman, Christopher B. Little, V. Ravi, Amanda J. Fosang, Constanza Angelucci, Louise H. W. Kung, Lynn Rowley, Katrina M. Bell

To explore the role of microRNAs in osteoarthritis (OA), we conducted microRNA expression profiling on micro-dissected tibial cartilage and subchondral bone in a mouse model of OA produced by medial meniscus destabilization (DMM). DMM mice had charac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17a98832b1bbd0b9c0f0aa37db8e52b1
https://doi.org/10.1101/113456