Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Louisa Tsai"'
Autor:
Louisa Tsai, Salman Azhar, Satyanarayana Medicherla, Yasmine Chandrasekher, Eve Reaven, Linda C. Giudice
Publikováno v:
The Journal of Clinical Endocrinology & Metabolism. 83:983-991
This study examines the ability of human high density lipoproteins (HDL3) to deliver cholesteryl esters to human granulosa cells and describes the selective cholesterol pathway by which this occurs. Luteinized cells obtained from subjects undergoing
Publikováno v:
Journal of Biological Chemistry. 271:16208-16217
The current study utilizes human, apoE-free high density lipoprotein reconstituted with a highly specific fluorescent-cholesteryl ester probe to define the initial steps and regulatory sites associated with the "selective" uptake and intracellular it
Autor:
Marjorie Spicher, Lotan Shilo, Salman Azhar, Louisa Tsai, Mohan Philip, Allen D. Cooper, Eve Reaven
Publikováno v:
Journal of Cellular Physiology. 161:449-462
Previous studies have shown that the B/E (low density lipoprotein [LDL]) receptor pathway plays a minor role in cholesterol uptake in the intact rat ovary, but when granulosa cells are isolated and maintained in culture, the cells develop a fully fun
Publikováno v:
Journal of Lipid Research, Vol 35, Iss 7, Pp 1161-1176 (1994)
This study examines various functional, biochemical, and structural changes in rat adrenocortical and ovarian granulosa cells that could account for the decline in lipoprotein-supported hormone production after cell treatment with the protein phospha
Publikováno v:
Biochemical and Biophysical Research Communications. 179:726-733
Rat adrenocortical cells in culture respond to stimulation by ACTH alone (15 fold over basal) and to ACTH + added lipoproteins (as an exogeneous source of cholesterol), with an additional 25–30 fold rise in steroidogenesis. With the addition of oka
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1047:148-160
Earlier studies have shown that rat granulosa cells grown in serum-free medium are exquisitely responsive to exogenously provided lipoprotein cholesterol. In this study we compare the amount of cholesterol (cholesteryl ester) actually delivered from
Cholesterol uptake by the 'selective' pathway of ovarian granulosa cells: early intracellular events
Publikováno v:
Journal of Lipid Research, Vol 36, Iss 7, Pp 1602-1617 (1995)
Although the ‘selective’ pathway is a major cholesteryl ester (CE) uptake pathway used by steroidogenic cells, essentially nothing is known about the itinerary of the CE once it is extracted from lipoproteins at the cell surface. In the current r
Publikováno v:
Journal of Lipid Research, Vol 29, Iss 7, Pp 869-882 (1988)
Recent findings from this laboratory have led to the suggestion that the hormone-producing cells of the rat luteinized ovary in situ may obtain a large share of low density lipoprotein (LDL) cholesterol without actually internalizing the intact lipop
Publikováno v:
Biochimica et biophysica acta. 963(2)
We have developed a chemically defined, serum-free medium for the culture of rat granulosa cells. This medium contains Dulbecco's modified Eagle's medium/Ham's nutrient F12 (DME:F12) (1:1) plus insulin (2 micrograms/ml), hydrocortisone (100 ng/ml), t