Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes

Autor: Maria Serrano-Albal, Marie Claire Aquilina, Lucas G. Kiazim, Louisa J. Zak, Darren K. Griffin, Peter J. Ellis

Publikováno v: Animals, Vol 14, Iss 17, p 2544 (2024)

Porcine in vitro embryo production (IVP) protocols have conventionally used density gradient selection (DGS) by centrifugation to prepare sperm samples and achieve successful fertilisation. However, the possible toxicity of the solutions used and the

Externí odkaz: https://doaj.org/article/80df3cdc89d64beaa24f32469ca0a5cc

Morphokinetic Profiling Suggests That Rapid First Cleavage Division Accurately Predicts the Chances of Blastulation in Pig In Vitro Produced Embryos

Autor: Lucy M. Hillyear, Louisa J. Zak, Tom Beckitt, Darren K. Griffin, Simon C. Harvey, Katie E. Harvey

Publikováno v: Animals, Vol 14, Iss 5, p 783 (2024)

The study of pig preimplantation embryo development has several potential uses: from agriculture to the production of medically relevant genetically modified organisms and from rare breed conservation to acting as a physiologically relevant model for

Externí odkaz: https://doaj.org/article/d62e343652584c71af2f6c9a2d849935

Preimplantation Genetic Testing for Aneuploidy (PGT-A) Reveals High Levels of Chromosomal Errors in In Vivo-Derived Pig Embryos, with an Increased Incidence When Produced In Vitro

Autor: Reina Jochems, Carla Canedo-Ribeiro, Giuseppe Silvestri, Martijn F. L. Derks, Hanne Hamland, Louisa J. Zak, Egbert F. Knol, Alan H. Handyside, Eli Grindflek, Darren K. Griffin

Publikováno v: Cells, Vol 12, Iss 5, p 790 (2023)

Preimplantation genetic testing for aneuploidy (PGT-A) is widespread, but controversial, in humans and improves pregnancy and live birth rates in cattle. In pigs, it presents a possible solution to improve in vitro embryo production (IVP), however, t

Externí odkaz: https://doaj.org/article/ca5b2eb7f5d74dc3a29518aa0593b802

Supplementation of porcine

Autor: María, Serrano Albal, Giuseppe, Silvestri, Lucas G, Kiazim, Lucy M, Vining, Louisa J, Zak, Grant A, Walling, Alexandra M, Haigh, Simon C, Harvey, Katie E, Harvey, Darren K, Griffin

Publikováno v: Zygote (Cambridge, England). 30(6)

In porcine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9c985394a7598e8ea216bf760d52fbc0
https://pubmed.ncbi.nlm.nih.gov/36047469

Follicular fluid steroid hormones and in vitro embryo development in Duroc and Landrace pigs

Autor: Reina Jochems, Ann Helen Gaustad, Bjarne Styrishave, Louisa J. Zak, Irma C. Oskam, Eli Grindflek, Frøydis D. Myromslien, Elisabeth Kommisrud, Anette K. Krogenæs

Publikováno v: Jochems, R, Gaustad, A H, Styrishave, B, Zak, L J, Oskam, I C, Grindflek, E, Myromslien, F D, Kommisrud, E & Krogenæs, A K 2022, ' Follicular fluid steroid hormones and in vitro embryo development in Duroc and Landrace pigs ', Theriogenology, vol. 190, pp. 15-21 . https://doi.org/10.1016/j.theriogenology.2022.07.004
Theriogenology

The Duroc sire line has a smaller litter size compared to the Landrace dam line and we have previously observed fewer surface follicles on Duroc ovaries one day after weaning. In that same study, a broader cumulus expansion and faster nuclear maturat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bf08fafeb6eb71260fedd97391c42d2
https://pubmed.ncbi.nlm.nih.gov/35863097