Functional and structural insights into the multi-step activation and catalytic mechanism of bacterial ExoY nucleotidyl cyclase toxins bound to actin-profilin.

Autor: Magda Teixeira Nunes, Pascal Retailleau, Dorothée Raoux-Barbot, Martine Comisso, Anani Amegan Missinou, Christophe Velours, Stéphane Plancqueel, Daniel Ladant, Undine Mechold, Louis Renault

Publikováno v: PLoS Pathogens, Vol 19, Iss 9, p e1011654 (2023)

ExoY virulence factors are members of a family of bacterial nucleotidyl cyclases (NCs) that are activated by specific eukaryotic cofactors and overproduce cyclic purine and pyrimidine nucleotides in host cells. ExoYs act as actin-activated NC toxins.

Externí odkaz: https://doaj.org/article/e8d27ca0bacb4de3a745bbb0ed4ad4ac

Bacterial Nucleotidyl Cyclases Activated by Calmodulin or Actin in Host Cells: Enzyme Specificities and Cytotoxicity Mechanisms Identified to Date

Autor: Magda Teixeira-Nunes, Pascal Retailleau, Martine Comisso, Vincent Deruelle, Undine Mechold, Louis Renault

Publikováno v: International Journal of Molecular Sciences, Vol 23, Iss 12, p 6743 (2022)

Many pathogens manipulate host cell cAMP signaling pathways to promote their survival and proliferation. Bacterial Exoenzyme Y (ExoY) toxins belong to a family of invasive, structurally-related bacterial nucleotidyl cyclases (NC). Inactive in bacteri

Externí odkaz: https://doaj.org/article/317863b6b0e84c1498eee3d8aa94d35b

Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin.

Autor: Dorothée Raoux-Barbot, Alexander Belyy, Lina Worpenberg, Sabrina Montluc, Celia Deville, Véronique Henriot, Christophe Velours, Daniel Ladant, Louis Renault, Undine Mechold

Publikováno v: PLoS ONE, Vol 13, Iss 11, p e0206133 (2018)

Several bacterial pathogens produce nucleotidyl cyclase toxins to manipulate eukaryotic host cells. Inside host cells they are activated by endogenous cofactors to produce high levels of cyclic nucleotides (cNMPs). The ExoY toxin from Pseudomonas aer

Externí odkaz: https://doaj.org/article/8dc762d320cd495f99a7a21786bb3364

The extreme C terminus of the Pseudomonas aeruginosa effector ExoY is crucial for binding to its eukaryotic activator, F-actin

Autor: Daniel Ladant, Ignacio Santecchia, Blandine Bourigault, Alexander Belyy, Undine Mechold, Louis Renault

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, 2018, 293 (51), pp.19785--19796. ⟨10.1074/jbc.RA118.003784⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2018, 293 (51), pp.19785--19796. ⟨10.1074/jbc.RA118.003784⟩

International audience; Bacterial nucleotidyl cyclase toxins are potent virulence factors that upon entry into eukaryotic cells are stimulated by endogenous cofactors to catalyze the production of large amounts of 35-cyclic nucleoside monophosphates.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9107b1871b65b749e804a92c5556c37f
https://hal.science/hal-02176494

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: a minireview

Autor: Undine Mechold, Daniel Ladant, Alexander Belyy, Louis Renault

Publikováno v: Toxicon
Toxicon, Elsevier, 2017, ⟨10.1016/j.toxicon.2017.12.046⟩
Toxicon, 2017, ⟨10.1016/j.toxicon.2017.12.046⟩
Toxicon, Elsevier, 2017, 〈10.1016/j.toxicon.2017.12.046〉

International audience; ExoY is one of four well-characterized Pseudomonas aeruginosa type 3 secretion system (T3SS) effectors. It is a nucleotidyl cyclase toxin that is inactive inside the bacteria, but becomes potently activated once it is delivere

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8168d8240bd9671c562fb52febbb1e65
https://hal-pasteur.archives-ouvertes.fr/pasteur-01765203