Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Lorne R. Palmer"'
Autor:
Mark Wood, Lorne R. Palmer, Emily P. Thi, David Fraser, Sara A. Majeski, James Heyes, Agnes Jarosz, Kieu Lam, Adam Judge, Amy C.H. Lee, Alan D. Martin, Xin Ye, Kevin McClintock, Holland Richard J
Publikováno v:
Molecular Therapy. 29:2910-2919
N-Acetylgalactosamine (GalNAc) conjugated short interfering RNAs (siRNAs) are a leading RNA interference (RNAi) platform allowing targeted inhibition of disease-causing genes in hepatocytes. More than a decade of development has recently resulted in
Autor:
Xiaohe Wang, Rose Kowalski, Holland Richard J, Lorne R. Palmer, Michael J. Sofia, Andrzej Ardzinski, Hui Huang, Emily P. Thi, Andrew S. Kondratowicz, Amy C.H. Lee, Xin Ye, Chris Pasetka, Holly M. Micolochick Steuer, Andrea Cuconati, Rene Rijnbrand
Publikováno v:
Journal of Hepatology. 70:e471
Publikováno v:
Molecular Therapy. 15:713-720
Using a new controlled mixing process, highly transfection-competent polyplexes were formed and subsequently encapsulated within a lipid bilayer. The resulting "pre-condensed stable plasmid lipid particles" (pSPLPs) have small size (104+/-3 nm) and l
Autor:
Ivanka Toudjarska, Jürgen Soutschek, Vandana Sood, Kevin McClintock, Akin Akinc, Birgit Bramlage, Matthew N. Fedoruk, Timothy Racie, Adam Judge, Ian MacLachlan, Hans-Peter Vornlocher, Lorne R. Palmer, Lloyd Jeffs, Kieu Lam, Sumi Shanmugam, William Zedalis, Tracy Zimmermann, Ingo Röhl, Matthias John, Ed Yaworski, Jens Harborth, Amy C.H. Lee, Muthiah Manoharan, Stephan Seiffert, James Heyes, David Bumcrot, Amanda J. Wheat, Victor Koteliansky, Lubomir Nechev
Publikováno v:
Nature. 441:111-114
The opportunity to harness the RNA interference (RNAi) pathway to silence disease-causing genes holds great promise for the development of therapeutics directed against targets that are otherwise not addressable with current medicines. Although there
Autor:
Angela M. Lam, David B. Fenske, Ammen P. Sandhu, Lorne R. Palmer, Pieter R. Cullis, Michael V. Johnston
Publikováno v:
Analytical Biochemistry. 341:156-164
Previous work from this laboratory has shown that plasmid DNA can be encapsulated in small (70-nm-diameter) stabilized plasmid–lipid particles (SPLP) that consist of a single plasmid encapsulated within a bilayer lipid vesicle. SPLP preferentially
Autor:
Lloyd Jeffs, Ellen Grace Ambegia, Ian MacLachlan, Cory Giesbrecht, Lorne R. Palmer, Shannon Ewanick
Publikováno v:
Pharmaceutical Research. 22:362-372
A fully scalable and extrusion-free method was developed to prepare rapidly and reproducibly stabilized plasmid lipid particles (SPLP) for nonviral, systemic gene therapy. Liposomes encapsulating plasmid DNA were formed instantaneously by mixing lipi
Publikováno v:
Journal of Liposome Research. 14:155-173
Cationic poly(ethylene glycol)-lipid conjugates (CPLs), a class of lipid designed to enhance the interaction of liposomes with cells, possess the following architectural features: 1) a hydrophobic lipid anchor of distearoylphosphatidylethanolamine (D
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1512(2):259-272
This paper describes a new method for enhancing the interaction of liposomes with cells. A novel class of cationic poly(ethyleneglycol) (PEG)-lipid (CPL) conjugates have been characterized for their ability to insert into pre-formed vesicles and enha
Autor:
Kevin McClintock, Catherine Choi, Lorne R. Palmer, Adam Judge, Ellen Grace Ambegia, Ian MacLachlan, Marjorie Robbins, Ed Yaworski
Publikováno v:
Human gene therapy. 19(10)
Activation of innate immunity has direct effects in modulating viral replication, tumor growth, angiogenesis, and inflammatory and other immunological processes. It is now established that unmodified siRNA can activate this innate immune response and
Publikováno v:
Bioconjugate chemistry. 11(3)
The synthesis of a new class of fluorescent cationic poly(ethylene glycol) lipid conjugates (CPLs) is described. These lipids consist of a hydrophobic distearoyl-phosphatidylethanolamine (DSPE) anchor coupled to a highly fluorescent N(epsilon)-dansyl