Zobrazeno 1 - 10
of 54
pro vyhledávání: '"Lorne B. Taichman"'
Publikováno v:
Journal of Investigative Dermatology Symposium Proceedings. 9(3):269-275
For long-term cutaneous gene therapy, the therapeutic gene must be targeted to stem cells and be stably transmitted to and expressed in descendant cells. Retroviral vectors are highly efficient in gene transfer to human keratinocyte stem cells in cul
Publikováno v:
Gene Therapy. 9:1278-1285
A number of genetic disorders are manifested in cutaneous epithelium and gene therapy approaches for treatment of such diseases are being considered. A successful gene therapy protocol requires durable and correctly targeted gene expression within th
Autor:
Soosan Ghazizadeh, Lorne B. Taichman
Publikováno v:
The EMBO Journal. 20:1215-1222
Continuous renewal of the epidermis and its appendages throughout life depends on the proliferation of a distinct population of cells called stem cells. We have used in situ retrovirus-mediated gene transfer to genetically mark cutaneous epithelial s
Publikováno v:
Journal of Investigative Dermatology. 111:492-496
Direct transfer of new genetic information to keratinocytes in epidermis may prove effective in treating certain genodermatoses; however, current methods for in vivo gene transfer to skin do not lead to persistence of the transgene. The goal of this
Autor:
Richard A. Morgan, Thomas G. Jensen, Daniel M. Sullivan, Lorne B. Taichman, R. Michael Blaese, Robert B. Nussenblatt, Karl G. Csaky
Publikováno v:
Human Gene Therapy. 8:2125-2132
Gyrate atrophy is a progressive blindness associated with deficiency of ornithine aminotransferase (OAT). The strategy of using an autologous keratinocyte graft, modified to express high levels of OAT as an ornithine-catabolizing skin-based enzyme si
Publikováno v:
Human Gene Therapy. 8:911-917
Disorders in which there is toxic buildup of circulating substrate may be treated by furnishing an enzyme reservoir capable of metabolically processing the excess substrate. The epidermal keratinocyte is a potential site for such a reservoir. In this
Publikováno v:
Virus Research. 43:85-90
Human papillomavirus type 11 (HPV11) normally infects keratinocytes of stratified epithelia and replicates as an episome. To untangle early events in the development of a papilloma, we have infected human keratinocytes with human papillomavirus type
Publikováno v:
Journal of Dental Research. 75:912-918
Re-epithelialization involves interactions between keratinocytes and the extracellular matrix upon which these cells move. It is hypothesized that keratinocytes are activated when wounded, and the resultant phenotypic change directs re-epithelializat
Publikováno v:
Virology. 212(2):285-294
Respiratory tract tissues containing latent human papillomavirus (HPV) 11 were analyzed by reverse transcription-polymerase chain reaction for the presence of viral-specific RNA from the early region of the genome and compared to a similar analysis o
Publikováno v:
Journal of Investigative Dermatology. 102(1):61-66
Recently it has been shown that apolipoprotein E (apoE) secreted by keratinocytes in transplanted epidermal grafts reaches the systemic circulation. In this study we ask which cells in cultures of epidermal keratinocytes, basal or suprabasal, are the