Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Lorna K. Jost"'
Publikováno v:
Biology of Reproduction. 69:1730-1735
Teratospermia (>60% of morphologically abnormal spermatozoa) is well documented in felids. Even morphologically normal spermatozoa from teratospermic ejaculates have reduced ability to undergo tyrosine phosphorylation, acrosome react, and bind and pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67f13e979afe464ab33a0f83bcb5d4d6
https://doi.org/10.1095/biolreprod.103.016089
https://doi.org/10.1095/biolreprod.103.016089
Autor:
Lorna K. Jost, Donald P. Evenson
Publikováno v:
Methods in Cell Science. 22:169-189
The Sperm Chromatin Structure Assay (SCSA) serves as a tool for measuring clinically important properties of sperm nuclear chromatin integrity. The assay utilizes the metachromatic features of Acridine Orange (AO), a DNA probe, and the principles of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55ba5760aff6b8ed6ab3b6e99917a39a
https://doi.org/10.1023/a:1009844109023
https://doi.org/10.1023/a:1009844109023
Publikováno v:
Human Reproduction. 14:2015-2019
The ability of double-layered density gradient centrifugation (DGC) or glass wool filtration (GWF) of semen to remove spermatozoa with damaged chromatin structure was assessed by the flow cytometric sperm chromatin structure assay (SCSA), which measu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48901edb3d6c62b5ff7282a5bee92a2c
https://doi.org/10.1093/humrep/14.8.2015
https://doi.org/10.1093/humrep/14.8.2015
Publikováno v:
Experimental Cell Research. 236:231-237
Susceptibility of mammalian sperm DNA to low pH- or heat-induced denaturation in situ has shown very strong dose-response relationships with animal and human exposure to chemical and physical toxicants and also fertility potential. In this study, 23
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3c3c2c5e75ed97e79887876fa98f2ab
https://doi.org/10.1006/excr.1997.3719
https://doi.org/10.1006/excr.1997.3719
Publikováno v:
Cytometry. 24:167-173
This study investigated the relationship between morphologically abnormal sperm, sperm chromatin structure, and fertility. Semen samples were obtained from 13 bulls. The sperm chromatin structure assay (SCSA), a sensitive measure of denaturability of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cb348fccc5f6109bde81de825ce86cf8
https://doi.org/10.1002/(sici)1097-0320(19960601)24:2<167::aid-cyto9>3.0.co
https://doi.org/10.1002/(sici)1097-0320(19960601)24:2<167::aid-cyto9>3.0.co
Autor:
Richard Coico, Tim Yopp, Kevin Becker, Ole Petter F. Clausen, Donald P. Evenson, Lori Rhodes, Donna Gandour, Lorna K. Jost, Andrew Daley, Paula M. De Angelis, Barbara Stanton
Publikováno v:
Cytometry. 19:295-303
The sperm chromatin structure assay (SCSA) measures the susceptibility of sperm nuclear DNA to acid-induced denaturation in situ, and was developed on two Ortho flow cytometers, an FC200 and a cytofluorograf 30 (BDIS), both having orthogonal axes of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e21024955e0ffa38e3bf2b5239a5634b
https://doi.org/10.1002/cyto.990190403
https://doi.org/10.1002/cyto.990190403
Publikováno v:
Biology of Reproduction. 52:655-659
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::342ae5c482f8b8089e9afcb9b91f41fd
https://doi.org/10.1093/biolreprod/52.monograph_series1.655
https://doi.org/10.1093/biolreprod/52.monograph_series1.655
Publikováno v:
Environmental and Molecular Mutagenesis. 25:23-30
The testicular regions of male mice were exposed to x-ray doses ranging from 0 to 400 rads. Forty days after exposure the mice were killed and the testes and cauda epididymal sperm removed surgically. Flow cytometric measurements of acridine orange s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e36d466cdbbe3396ffd58c15f4980eb
https://doi.org/10.1002/em.2850250105
https://doi.org/10.1002/em.2850250105
Publikováno v:
Environmental and Molecular Mutagenesis. 21:144-153
Effects of methyl methanesulfonate (MMS) on mouse testicular cell kinetics and sperm chromatin structure were determined flow cytometrically. Mice were exposed to a single ip injection of saline containing 0 or 150 mg/kg MMS, Relative ratios of 1N, 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e925c12014348b20a5f5f97a15617a1c
https://doi.org/10.1002/em.2850210208
https://doi.org/10.1002/em.2850210208
Autor:
Donald P. Evenson, Lorna K. Jost
Publikováno v:
Current Protocols in Cytometry
The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. Damaged DNA in the single sperm that fertilizes the female egg can have a dramatic negative impact on fetal development. This compr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::907b7887ae586cfbbac9b5a331820802
https://pubmed.ncbi.nlm.nih.gov/18770725
https://pubmed.ncbi.nlm.nih.gov/18770725