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pro vyhledávání: '"Lori E. Andrews"'
Publikováno v:
Methods. 32:428-436
An allosteric ribozyme is an RNA-based enzyme (ribozyme) whose catalytic activity is modulated by molecular recognition of a protein. The direct coupling of a detectable catalytic event to molecular recognition by an allosteric ribozyme enables simpl
Autor:
James McSwiggen, Scott D. Seiwert, Barry Polisky, Narendra K. Vaish, Christopher Pasko, Vasant Jadhav, Karl Kossen, Lori E. Andrews
Publikováno v:
RNA. 9:1058-1072
We describe a strategy for the ultra-sensitive detection of nucleic acids using “half” ribozymes that are devoid of catalytic activity unless completed by a trans-acting target nucleic acid. The half-ribozyme concept was initially demonstrated us
Autor:
Michele Richardson, Anna M. Gallegos, Francine E. Wincott, Arun B. Agrawal, Lori E. Andrews, Cynthia Cushman, Tom J. Parry, Victor Mokler, Pamela Pavco, Lara Maloney
Publikováno v:
Nucleic Acids Research. 27:2569-2577
Vascular endothelial growth factor (VEGF) and its receptors Flt-1 and KDR play important roles in physiological and pathological angiogenesis. Ribozymes that target the VEGF receptor mRNAs were developed and their biological activities in cell cultur
Autor:
Lawrence M. Blatt, Lori E. Andrews, Scott D. Seiwert, Narendra K. Vaish, Natalie G. Ahn, Rebecca E. Schweppe, Fang Dong
Publikováno v:
Nature biotechnology. 20(8)
An allosteric hammerhead ribozyme activated specifically by the unphosphorylated form of the protein kinase ERK2 was created through a rational design strategy that relies on molecular recognition of ERK2 to decrease the formation of an alternate, in
Autor:
Jenny Fichmann, Diana M. Smith, Alma L. Burlingame, B M D Lois Epstein, Neil M. Matsui, Christine M. Sullivan, Karl R. Clauser, Lori E. Andrews
Publikováno v:
Electrophoresis. 18(3-4)
Two-dimensional (2-D) polyacrylamide gel electrophoresis combined with mass spectrometry is a powerful combination of technologies that allows high resolution separation of proteins and their rapid identification. Immobilized pH gradient (IPG) first-
Autor:
Huu M. Tran, Karl R. Clauser, Diana M. Smith, Fred C. Walls, Alma L. Burlingame, Lori E. Andrews, Steven C. Hall, James W. Webb, Lois B. Epstein
Publikováno v:
Mass Spectrometry in the Biological Sciences ISBN: 9781461266716
For twenty years two-dimensional polyacrylamide gel electrophoresis (2D PAGE) has been used to simultaneously separate and visualize by suitable staining, hundreds of proteins present in cell lysates [1, 2]. This powerful methodology allows the creat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e1d56c26aa6d2f67cc9ecbcebd4fefa1
https://doi.org/10.1007/978-1-4612-0229-5_9
https://doi.org/10.1007/978-1-4612-0229-5_9
Autor:
Lori E. Andrews, James W. Webb, Alma L. Burlingame, Diana M. Smith, Huu M. Tran, Steven C. Hall, Karl R. Clauser, Lois B. Epstein
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 92(11)
We report a general mass spectrometric approach for the rapid identification and characterization of proteins isolated by preparative two-dimensional polyacrylamide gel electrophoresis. This method possesses the inherent power to detect and structura