Výsledky vyhledávání - "Loreen Ehrlich"

Akademický článek

Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Autor: Alexander Paping, Clara Basler, Rebecca C Rancourt, Loreen Ehrlich, Kerstin Melchior, Wolfgang Henrich, Thorsten Braun

Publikováno v: BioTechniques, Vol 72, Iss 6, Pp 273-278 (2022)

Samples for histological analyses are often formalin-fixed paraffin-embedded (FFPE) and slide-mounted, which complicates RNA extraction for many downstream molecular applications. Furthermore, when the region of interest is extremely small due to iso

Externí odkaz: https://doaj.org/article/faf632bbaab143e9ba7f1a01179e58cc

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Akademický článek

Detection of Microplastic in Human Placenta and Meconium in a Clinical Setting

Autor: Thorsten Braun, Loreen Ehrlich, Wolfgang Henrich, Sebastian Koeppel, Ievgeniia Lomako, Philipp Schwabl, Bettina Liebmann

Publikováno v: Pharmaceutics, Vol 13, Iss 7, p 921 (2021)

Environmental pollution with microplastics (MPs) is a major and worldwide concern. Involuntary exposure to MPs by ingestion or inhalation is unavoidable. The effects on human health are still under debate, while in animals, cellular MP translocation

Externí odkaz: https://doaj.org/article/800f17af0de3454f900e3a268626b5d6

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Intraoperative ultrasound during repeat cesarean delivery facilitates sampling of uterine scar tissue

Autor: Alexander Paping, Clara Basler, Kerstin Melchior, Loreen Ehrlich, Mario Thiele, Georg N. Duda, Wolfgang Henrich, Thorsten Braun

Publikováno v: Journal of perinatal medicineReferences.

Objectives Histological examination of uterine scars provides insight into uterine wound healing and helps to develop prevention methods of uterine wall rupture after previous uterine surgery. Therefore, exact intraoperative scar identification is ne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80a61958d5cd116727484535ac0b2345
https://pubmed.ncbi.nlm.nih.gov/36394552

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Optimized protocol for the RNA isolation of laser microdissected formalin-fixed paraffin-embedded uterine scar tissues for RNA expression analyses v1

Autor: Alexander Paping, Clara Basler, Rebecca C. Rancourt, Loreen Ehrlich, Kerstin Melchior, Wolfgang Henrich, Thorsten Braun

Samples for histological analyses are often formalin-fixed paraffin-embedded (FFPE) and slide-mounted. This complicates RNA extraction for molecular downstream applications. Additionally, when the region of interest is limited to a smaller area with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::30355296a75d3e297a30b7f8b4732bb1
https://doi.org/10.17504/protocols.io.byhgpt3w

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The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta

Autor: F. Braun, Deborah M. Sloboda, H. Gütling, Massimo Bionaz, Thorsten Braun, Wolfgang Henrich, Loreen Ehrlich, AK Gramzow, Andreas Plagemann

Publikováno v: Placenta. 44

RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. R

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e1c5384103b4976a9ed83bd48636d62
https://pubmed.ncbi.nlm.nih.gov/27452434

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Effects of Maternal Dexamethasone Treatment Early in Pregnancy on Glucocorticoid Receptors in the Ovine Placenta

Autor: Wolfgang Henrich, Andreas Plagemann, John Challis, Thorsten Braun, Hongkai Shang, Joachim W. Dudenhausen, Wenbin Meng, John P. Newnham, Deborah M. Sloboda, Shaofu Li, Loreen Ehrlich

The effects of endogenous cortisol on binucleate cells (BNCs), which promote fetal growth, may be mediated by glucocorticoid receptors (GRs), and exposure to dexamethasone (DEX) in early pregnancy stages of placental development might modify this res

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed987ca1f62d12d0f45497f1d6ad6186
https://europepmc.org/articles/PMC4519764/

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The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Autor: Andreas Plagemann, Massimo Bionaz, John P. Newnham, Thorsten Braun, Deborah M. Sloboda, Huaisheng Xu, John R. G. Challis, Shaofu Li, Loreen Ehrlich, Joachim W. Dudenhausen, Wolfgang Henrich

Publikováno v: BMC Research Notes

Background The key to understanding changes in gene expression levels using reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) relies on the ability to rationalize the technique using internal control genes (ICGs). Howev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::072383db95225393a56a1b02bb3a6e49
https://doi.org/10.1186/s13104-015-0973-7

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Early Dexamethasone Treatment Induces Placental Apoptosis in Sheep

Autor: Huaisheng Xu, Hongkai Shang, Andreas Plagemann, Karolin Lange, John R. G. Challis, John P. Newnham, Joachim W. Dudenhausen, Wolfgang Henrich, Thorsten Braun, Wenbin Meng, Deborah M. Sloboda, Shaofu Li, Loreen Ehrlich

Glucocorticoid treatment given in late pregnancy in sheep resulted in altered placental development and function. An imbalance of placental survival and apoptotic factors resulting in an increased rate of apoptosis may be involved. We have now invest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7b1da4eabb99982f27f12fa40d866de
https://europepmc.org/articles/PMC4275451/

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