Direct Plasmid DNA Transfection into Differentiated Mouse C2C12 Myotubes

Autor: Amanda Sales Conniff, Aneeshea Cason, Juliana Gonzalez Alvarado, Manya Bhandary, Shreena Patel, Julie Singh, Richard Heller, Loree C. Heller

Publikováno v: Re:GEN Open, Vol 4, Iss 1, Pp 1-8 (2024)

Background: Models of muscle cell culture allow simple manipulation to optimize gene transfer experiments, offering a significant opportunity to model muscle effects by allowing precise control of the cell culture environment in a cost-effective mann

Externí odkaz: https://doaj.org/article/e2e1260046754ded9ad3ab39116d0be1

Transcriptomic Analysis of the Acute Skeletal Muscle Effects after Intramuscular DNA Electroporation Reveals Inflammatory Signaling

Autor: Amanda Sales Conniff, Jared Tur, Kristopher Kohena, Min Zhang, Justin Gibbons, Loree C. Heller

Publikováno v: Vaccines, Vol 10, Iss 12, p 2037 (2022)

Skeletal muscle is a promising tissue for therapeutic gene delivery because it is highly vascularized, accessible, and capable of synthesizing protein for therapies or vaccines. The application of electric pulses (electroporation) enhances plasmid DN

Externí odkaz: https://doaj.org/article/e220f0b9fb4a448b90ae647bf68d5d35

Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells

Autor: Katarina Znidar, Masa Bosnjak, Maja Cemazar, Loree C. Heller

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 5, Iss C (2016)

In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of se

Externí odkaz: https://doaj.org/article/00fe8ab3831e45c680dbacec9c19d833

Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells

Autor: Reynald Thinard, Sezgi Arpag, Claude Nicolau, Melanie Chevalier, Loree C. Heller, Richard Heller, Yu Jing, Ruth Greferath

Publikováno v: Journal of Cellular and Molecular Medicine

In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood‐brain barrier (BBB) breakdown. After intravenous or intra‐arterial injection

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cea94814b95e59572d2efdc2d4569010
https://doi.org/10.1111/jcmm.15511

Radiation Induced Upregulation of DNA Sensing Pathways is Cell-Type Dependent and Can Mediate the Off-Target Effects

Autor: Katarina Znidar, Masa Bosnjak, Bostjan Markelc, Tanja Jesenko, Gregor Sersa, Maja Cemazar, Loree C. Heller

Publikováno v: Cancers
Volume 12
Issue 11
Cancers, str. 3365-1-3365-23, Vol. 12, 2020
COBISS-ID: 517914137
Cancers, vol. 12, pp. 3365-1-3365-23, 2020.
Cancers, Vol 12, Iss 3365, p 3365 (2020)

Irradiation of tumors generates danger signals and inflammatory cytokines that promote the off-target bystander and abscopal effects, evident especially when radiotherapy is administered in combination with the immune checkpoint inhibitors (ICI). The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::febf6a3a52c6f80f21c419f89a2752b7