Mapping fast DNA polymerase exchange during replication

Autor: Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)

Abstract Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like helicase. Here we present data, emp

Externí odkaz: https://doaj.org/article/3d5c500a721b415dbb08911b751ff116

Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches

Autor: Gijs Wuite, Longfu Xu, Matthew Halma

Publikováno v: Xu, L, Halma, M T J & Wuite, G J L 2023, ' Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches ', International Journal of Molecular Sciences, vol. 24, no. 3, 2806, pp. 1-32 . https://doi.org/10.3390/ijms24032806

Single-stranded DNA-binding proteins (SSBs) play vital roles in DNA metabolism. Proteins of the SSB family exclusively and transiently bind to ssDNA, preventing the DNA double helix from re-annealing and maintaining genome integrity. In the meantime,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::370d46df368e8d0b860015f86b34bd30
https://doi.org/10.3390/ijms24032806

Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain

Autor: Yongdong Liu, Yao Zhang, Liping Liu, Jian Wang, Longfu Xu, Qi Wang, Chun Zhang, Zhiguo Su

Publikováno v: Protein Expression and Purification. 139:14-20

Ciliary neurotrophic factor (CNTF) is a promising candidate for the treatment of neurodegenerative or metabolic diseases, but suffers rapid clearance in body. Herein we constructed a new long-acting recombinant human CNTF (rhCNTF) by genetic fusion w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e506cf07a1c93473fca8eba51a17e71
https://doi.org/10.1016/j.pep.2017.07.006

Purification and assembling a fused capsid protein as an enterovirus 71 vaccine candidate from inclusion bodies to pentamer-based nanoparticles

Autor: Zhiguo Su, Qi Wang, Chuan Qin, Ling Xue, Yongdong Liu, Jian Luo, Chun Zhang, Jiangning Liu, Jian Wang, Longfu Xu

Publikováno v: Biochemical Engineering Journal. 117:139-146

An efficient preparation process for a novel Enterovirus 71 (EV71) vaccine was developed in this paper, which is a fused antigen by connecting the truncated capsid proteins of VP1, VP2 and VP3 into one molecule through flexible peptide linkers and ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7155e61e551544fcbcf92741fcd229a1
https://doi.org/10.1016/j.bej.2016.10.009

Single-Molecule Movies of the Interplay between DNA Polymerase and Single Strand DNA Binding (SSB) Protein

Autor: Erwin J.G. Peterman, Longfu Xu, Andreas S. Biebricher, Gijs J.L. Wuite, Julia A M Bakx, Seyda Acar

Publikováno v: Biophysical Journal. 116:75a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2c5e040abc1109008fc1bab042b28bf7
https://doi.org/10.1016/j.bpj.2018.11.444