Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Long ssDNA"'
Autor:
Akira Mabuchi, Shoji Hata, Mariya Genova, Chiharu Tei, Kei K. Ito, Masayasu Hirota, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-16 (2023)
Abstract Background Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR). For knock-
Externí odkaz:
https://doaj.org/article/ee096e6001b749df988891c536a3bf4d
Autor:
Deshani C. Ranawakage, Keita Okada, Kota Sugio, Yuya Kawaguchi, Yuki Kuninobu-Bonkohara, Takuya Takada, Yusuke Kamachi
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://doaj.org/article/0c8dc2dc68eb4a5ebc6159214f78efde
Autor:
Masato Ohtsuka, Masahiro Sato, Hiromi Miura, Shuji Takabayashi, Makoto Matsuyama, Takayuki Koyano, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy
Publikováno v:
Genome Biology, Vol 19, Iss 1, Pp 1-15 (2018)
Abstract We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single
Externí odkaz:
https://doaj.org/article/434525404bbe47ed92639ed52ed7de0a
Autor:
Rolen M. Quadros, Hiromi Miura, Donald W. Harms, Hisako Akatsuka, Takehito Sato, Tomomi Aida, Ronald Redder, Guy P. Richardson, Yutaka Inagaki, Daisuke Sakai, Shannon M. Buckley, Parthasarathy Seshacharyulu, Surinder K. Batra, Mark A. Behlke, Sarah A. Zeiner, Ashley M. Jacobi, Yayoi Izu, Wallace B. Thoreson, Lisa D. Urness, Suzanne L. Mansour, Masato Ohtsuka, Channabasavaiah B. Gurumurthy
Publikováno v:
Genome Biology, Vol 18, Iss 1, Pp 1-15 (2017)
Abstract Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice a
Externí odkaz:
https://doaj.org/article/cc09dd43b09b4403b9ad7415943adf1e
Akademický článek
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Autor:
Yukiko U. Inoue, Yuki Morimoto, Mayumi Yamada, Ryosuke Kaneko, Kazumi Shimaoka, Shinji Oki, Mayuko Hotta, Junko Asami, Eriko Koike, Kei Hori, Mikio Hoshino, Itaru Imayoshi, Takayoshi Inoue
Publikováno v:
Cells, Vol 10, Iss 5, p 1076 (2021)
Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions in vivo. Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically
Externí odkaz:
https://doaj.org/article/55a6df81cf8e4f539542750ba586a3e0
Autor:
Deshani C. Ranawakage, Takuya Takada, Yuki Kuninobu-Bonkohara, Yusuke Kamachi, Keita Okada, Yuya Kawaguchi, Kota Sugio
Publikováno v:
Frontiers in Cell and Developmental Biology
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c79710d470cd5a01fd666a2e43e4c8e9
https://pubmed.ncbi.nlm.nih.gov/33681181
https://pubmed.ncbi.nlm.nih.gov/33681181
Akademický článek
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Autor:
Yutaka Inagaki, Suzanne L. Mansour, Ronald Redder, Parthasarathy Seshacharyulu, Guy P. Richardson, Daisuke Sakai, Mark A. Behlke, Masato Ohtsuka, Hisako Akatsuka, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Lisa D. Urness, Tomomi Aida, Donald W. Harms, Takehito Sato, Wallace B. Thoreson, Shannon M. Buckley, Yayoi Izu, Sarah A. Zeiner, Hiromi Miura, Ashley M. Jacobi, Surinder K. Batra
Publikováno v:
Genome Biology, Vol 18, Iss 1, Pp 1-15 (2017)
Genome Biology
Genome Biology
Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are genera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f18d729e2d7fc752e6730f9c4382957e
http://link.springer.com/article/10.1186/s13059-017-1220-4
http://link.springer.com/article/10.1186/s13059-017-1220-4
Akademický článek
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