Multi-modal molecular imaging approaches to detect primary cells in preclinical models

Autor: Geraldine Escher, Juan Manuel Cardenas-Maestre, Lois M. Alexander, Neil Q. McDonald, Rosario M. Sánchez-Martín, Kevin Dhaliwal, Asier Unciti-Broceta, Mark Bradley, Christopher Haslett, Maurits A. Jansen, John Simpson

Publikováno v: Faraday Discuss.. 149:107-114

The need to understand cellular trafficking in vivo in situ requires the development and application of novel methodologies for cellular labeling and cell tracking. Here we applied new technologies associated with advances in molecular imaging to dem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fe36e107473e6b3b44089d6b8ee6614
https://doi.org/10.1039/c005410k

Microsphere-based tracing and molecular delivery in embryonic stem cells

Autor: Joshua M. Brickman, Nicole Gennet, Lois M. Alexander, Anestis Tsakiridis, Alessandra Livigni, Mark Bradley, Meng Li, Rosario M. Sánchez-Martín

Publikováno v: Biomaterials. 30:5853-5861

Embryonic stem (ES) cells are in vitro cell lines that can differentiate into all lineages of the fetus and the adult. Despite the versatility of genetic manipulation in murine ES cells, these approaches are time-consuming and rely on inefficient tra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b27e691d496987d30c4c9ea556d1daf9
https://doi.org/10.1016/j.biomaterials.2009.06.024

Knocking (Anti)-Sense into Cells: The Microsphere Approach to Gene Silencing

Autor: Rosario M. Sánchez-Martín, Lois M. Alexander, Mark Bradley

Publikováno v: Bioconjugate Chemistry. 20:422-426

200 and 500 nm polymeric microspheres have been conjugated to siRNA targeted against EGFP expressed in human cervical cancer (HeLa) cells and shown to efficiently silence protein expression over 72 h, without detrimental cytotoxicity. Furthermore, wi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdb27d0fe9e30e812e3ea8cff2899a72
https://doi.org/10.1021/bc800529r

Synthesis and characterization of dual-functionalized core-shell fluorescent microspheres for bioconjugation and cellular delivery

Autor: Mark Bradley, Anna V. Hine, David A. Nagel, Jonathan M. Behrendt, Damien Dupin, Andrew J. Sutherland, Lois M. Alexander, Evita Chundoo

Publikováno v: PLoS ONE
PLoS ONE, Vol 8, Iss 3, p e50713 (2013)
Behrendt, J M, Nagel, D, Chundoo, E, Alexander, L M, Dupin, D, Hine, A V, Bradley, M, Sutherland, A J & Bing, X (ed.) 2013, ' Synthesis and Characterization of Dual-Functionalized Core-Shell Fluorescent Microspheres for Bioconjugation and Cellular Delivery ', PLoS ONE, vol. 8, no. 3, e50713, pp.-. https://doi.org/10.1371/journal.pone.0050713

The efficient transport of micron-sized beads into cells, via a non-endocytosis mediated mechanism, has only recently been described. As such there is considerable scope for optimization and exploitation of this procedure to enable imaging and sensin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45c0e05b8456fa383124b1cf418626ba
https://publications.aston.ac.uk/id/eprint/39998/1/Dual_functionalized_core_shell_fluorescent_microspheres_for_bioconjugation_and_cellular_delivery.pdf

Multifunctionalized biocompatible microspheres for sensing

Autor: Lois M. Alexander, Mark Bradley, Rosario M. Sánchez-Martín

Publikováno v: Annals of the New York Academy of Sciences. 1130

We present our achievements in the synthesis and application of multifunctionalized, cross-linked, polystyrene microspheres, which remarkably and quite generally are taken up by all cell types studied to date. Importantly, the nature of these synthet

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe992d010b364582297c6f9bd2afea1e
https://pubmed.ncbi.nlm.nih.gov/18596350