Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Lluis Masip"'
Publikováno v:
Analytical and Bioanalytical Chemistry. 413:1929-1939
The immobilization of sensing bioreceptors is a critical feature affecting the final performance of a biosensor. For DNA detection, the (strept)avidin-biotin affinity interaction is often used for the immobilization of biotin-labeled oligonucleotides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1433482f208a30025878dbf6e80a5b2
https://doi.org/10.1007/s00216-021-03162-5
https://doi.org/10.1007/s00216-021-03162-5
Capture, detection and purification of dsDNA amplicons using a DNA binding protein on magnetic beads
Publikováno v:
Analytical Biochemistry. 658:114923
Magnetic separation has been widely exploited for capture and detection of nucleic acids, including amplicons. Streptavidin-magnetic beads (SA-MB) are typically employed for this purpose, as well as in biosensing applications. However, remaining biot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c69baeeb99e0b1ee1cefacc111c2475
https://doi.org/10.1016/j.ab.2022.114923
https://doi.org/10.1016/j.ab.2022.114923
Publikováno v:
Harmful algae. 110
The proliferation of harmful microalgae endangers aquatic ecosystems and can have serious economic implications on a global level. Harmful microalgae and their associated toxins also pose a threat to human health since they can cause seafood-borne di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4018208cb7179e98924d6f7a3a8f9b06
https://pubmed.ncbi.nlm.nih.gov/34887012
https://pubmed.ncbi.nlm.nih.gov/34887012
Publikováno v:
Harmful Algae. 110:102135
The proliferation of harmful microalgae endangers aquatic ecosystems and can have serious economic implications on a global level. Harmful microalgae and their associated toxins also pose a threat to human health since they can cause seafood-borne di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3436d3a6d272019965bd88d00fa24159
https://doi.org/10.1016/j.hal.2021.102135
https://doi.org/10.1016/j.hal.2021.102135
Nucleic acid lateral flow assays using a conjugate of a DNA binding protein and carbon nanoparticles
Publikováno v:
Microchimica acta 186 (2019) 7
Microchimica acta, 186(7)
Microchimica acta, 186(7)
Nucleic acid lateral flow assays (NALFA) are often performed with gold nanoparticles. These are typically associated with ligand-labeled PCR amplicons via affinity interactions of adsorbed/conjugated proteins. Otherwise, they are conjugated to specif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b86ec9b6ac377e7c425f7cd1ead1b55b
https://doi.org/10.1007/s00604-019-3544-0
https://doi.org/10.1007/s00604-019-3544-0
Publikováno v:
Analytical and bioanalytical chemistry. 411(16)
The use of aptamers in various analytical applications as molecular recognition elements and alternative to antibodies has led to the development of various platforms that facilitate the sensitive and specific detection of targets ranging from small
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6da4de286c45bd4461cd043cf38dd1b5
https://pubmed.ncbi.nlm.nih.gov/31089784
https://pubmed.ncbi.nlm.nih.gov/31089784
Publikováno v:
Biosensors and Bioelectronics. 74:1005-1010
The need for sensitive detection of DNA is growing as more specific DNA sequences are being correlated to gene markers for disease diagnosis, food safety and other security related applications. Detection in hybridization-based assays is usually achi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f75e2590d62056b4a43662a0a62c640c
https://doi.org/10.1016/j.bios.2015.07.077
https://doi.org/10.1016/j.bios.2015.07.077
Publikováno v:
Analytical and bioanalytical chemistry. 409(14)
A versatile and universal DNA sensing platform is presented based on enzyme-DNA binding protein tags conjugates and simple DNA nanostructures. Two enzyme conjugates were thus prepared, with horseradish peroxidase linked to the dimeric single-chain ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69bf2562b21437d2cd034b900e4f3d3b
https://pubmed.ncbi.nlm.nih.gov/28331958
https://pubmed.ncbi.nlm.nih.gov/28331958
Publikováno v:
Journal of Biological Chemistry. 283:840-848
Thioredoxin exported into the Escherichia coli periplasm catalyzes the oxidation of protein thiols in a DsbB-dependent function. However, the oxidative activity of periplasmic thioredoxin is insufficient to render dsbA(-) cells susceptible to infecti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce151ea9f62085a14b467ee7fb6089d9
https://doi.org/10.1074/jbc.m705147200
https://doi.org/10.1074/jbc.m705147200
Autor:
Sung Kun Kim, Masami Kusunoki, Masakazu Hirasawa, Toshiharu Hase, Hideaki Unno, José M. Gualberto, Lluis Masip, Michael K. Johnson, Sibali Bandyopadhyay, George Georgiou, Nicolas Rouhier, Virginie Lattard, David B. Knaff, Jean-Pierre Jacquot
Publikováno v:
Proceedings of the National Academy of Sciences. 104:7379-7384
When expressed in Escherichia coli , cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron–sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbca1620778c41d1dbc5c92347eac327
https://doi.org/10.1073/pnas.0702268104
https://doi.org/10.1073/pnas.0702268104