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Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autor: Hof, Lotta, Moreth, Till, Koch, Michael, Liebisch, Tim, Kurtz, Marina, Tarnick, Julia, Lissek, Susanna M., Verstegen, Monique M. A., van der Laan, Luc J. W., Huch, Meritxell, Matthäus, Franziska, Stelzer, Ernst H. K., Pampaloni, Francesco

Publikováno v: BMC Biology
BMC Biology, Vol 19, Iss 1, Pp 1-22 (2021)
BMC Biology, 19(1):37. BioMed Central Ltd.

Background Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective cell behaviour in mammalian organs during development, homeostasis, regeneration,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f4a92f031cdae4755e518ea978d990e6
http://europepmc.org/articles/PMC7903752

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Additional file 7 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autor: Hof, Lotta, Moreth, Till, Koch, Michael, Liebisch, Tim, Kurtz, Marina, Tarnick, Julia, Lissek, Susanna M., Verstegen, Monique M. A., Laan, Luc J. W. Van Der, Meritxell Huch, Matthäus, Franziska, Stelzer, Ernst H. K., Pampaloni, Francesco

Additional file 7: Fig. S6. Representative overview images of three different mPO cultures grown in Z.1-FEP-cuvettes. (a) mPO grown within the Z.1-FEP-cuvette were kept in an incubator as a control for organoids grown within the Z.1 microscope. Image

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Additional file 14 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 14: Fig. S13. Bright field pipeline allows detailed analysis of polycystic hCCAOs. (a) Polycystic hCCAOs display a dense phenotype. Microscope: Zeiss Axio Observer Z.1; objective lenses: Plan-Apochromat 5x/0.16, avg. z-projection, vox

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Additional file 3 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 3: Fig. S2. Ultra-thin FEP-foil cuvette holders for live recordings with the Zeiss Lightsheet Z.1 microscope system. (a) Illustration of the general setup of the Zeiss Lightsheet Z.1 microscope. (b) Close-up of the microscope chamber

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Additional file 5 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 5: Fig. S4. Validation of the pH properties of the Zeiss Lightsheet Z.1 microscope. (a) Illustration of the pH-value distribution inside the chamber of the Zeiss Lightsheet Z.1 microscope and the corresponding measurement landmarks. A

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Additional file 23 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 23: Table S1. Used settings for the segmentation and post-processing of the data obtained with the light-sheet pipeline. Table S2. Evaluation of segmentation performance for different organoids. The performance was measured against a

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Additional file 8 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 8: Fig. S7. Dynamic processes observed in organoid morphogenesis. hCCAOs expressed the nuclei marker H2B-eGFP (magenta) and the F-actin cytoskeletal marker LifeAct-mCherry (green) and were cultured in Z1-FEP-cuvettes for long-term liv

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Additional file 4 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 4: Fig. S3. Validation of the temperature properties of the Zeiss Lightsheet Z.1 microscope. (a) Illustration of the temperature distribution inside of the Zeiss Lightsheet Z.1 microscope chamber and the corresponding measurement land

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Additional file 6 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 6: Fig. S5. Overview of entire hCCAO cultures within one Z1-FEP-cuvette and observation of isolated single-cell dynamics. hCCAOs expressed the nuclei marker H2B-eGFP (magenta) and the F-actin cytoskeletal marker LifeAct-mCherry (green

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bdb5aebbdff3a79892574636269699e

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