Výsledky vyhledávání - "Lissek, Susanna M"

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Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autor: Hof, Lotta, Moreth, Till, Koch, Michael, Liebisch, Tim, Kurtz, Marina, Tarnick, Julia, Lissek, Susanna M., Verstegen, Monique M. A., van der Laan, Luc J. W., Huch, Meritxell, Matthäus, Franziska, Stelzer, Ernst H. K., Pampaloni, Francesco

Publikováno v: BMC Biology
BMC Biology, Vol 19, Iss 1, Pp 1-22 (2021)
BMC Biology, 19(1):37. BioMed Central Ltd.

Background Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective cell behaviour in mammalian organs during development, homeostasis, regeneration,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f4a92f031cdae4755e518ea978d990e6
http://europepmc.org/articles/PMC7903752

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Additional file 11 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autor: Hof, Lotta, Moreth, Till, Koch, Michael, Liebisch, Tim, Kurtz, Marina, Tarnick, Julia, Lissek, Susanna M., Verstegen, Monique M. A., Laan, Luc J. W. Van Der, Meritxell Huch, Matthäus, Franziska, Stelzer, Ernst H. K., Pampaloni, Francesco

Additional file 11: Fig. S10. Illustration of the input, the assumptions and the output of the model. Measured cell counts and cell division dynamics are used to initialise the simulations. Organoid behaviour is based on the following main assumption

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87949dbda52960e77a198a54ea9489e3

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Additional file 8 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 8: Fig. S7. Dynamic processes observed in organoid morphogenesis. hCCAOs expressed the nuclei marker H2B-eGFP (magenta) and the F-actin cytoskeletal marker LifeAct-mCherry (green) and were cultured in Z1-FEP-cuvettes for long-term liv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46e6bd1684e278e9b4346fd706c9eb5c

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Additional file 4 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 4: Fig. S3. Validation of the temperature properties of the Zeiss Lightsheet Z.1 microscope. (a) Illustration of the temperature distribution inside of the Zeiss Lightsheet Z.1 microscope chamber and the corresponding measurement land

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49905e7129cc6d91f0841b07088e05b1

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Additional file 7 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 7: Fig. S6. Representative overview images of three different mPO cultures grown in Z.1-FEP-cuvettes. (a) mPO grown within the Z.1-FEP-cuvette were kept in an incubator as a control for organoids grown within the Z.1 microscope. Image

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Additional file 3 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 3: Fig. S2. Ultra-thin FEP-foil cuvette holders for live recordings with the Zeiss Lightsheet Z.1 microscope system. (a) Illustration of the general setup of the Zeiss Lightsheet Z.1 microscope. (b) Close-up of the microscope chamber

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Additional file 5 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 5: Fig. S4. Validation of the pH properties of the Zeiss Lightsheet Z.1 microscope. (a) Illustration of the pH-value distribution inside the chamber of the Zeiss Lightsheet Z.1 microscope and the corresponding measurement landmarks. A

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Additional file 23 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Additional file 23: Table S1. Used settings for the segmentation and post-processing of the data obtained with the light-sheet pipeline. Table S2. Evaluation of segmentation performance for different organoids. The performance was measured against a

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