Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo

Autor: Lisitskaya, Lidiya, Kropocheva, Ekaterina, Agapov, Aleksei, Prostova, Maria, Panteleev, Vladimir, Yudin, Denis, Ryazansky, Sergei, Kuzmenko, Anton, Aravin, Alexei A., Esyunina, Daria, Kulbachinskiy, Andrey

Prokaryotic Argonaute proteins (pAgos) are homologs of eukaryotic Argonautes (eAgos) and are also thought to play a role in cell defense against invaders. However, pAgos are much more diverse than eAgos and little is known about their functional acti

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Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo.

Autor: Lisitskaya L; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Kropocheva E; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Agapov A; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Prostova M; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Panteleev V; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia.; Moscow Institute of Physics and Technology, Dolgoprudny141700, Russia., Yudin D; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Ryazansky S; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Kuzmenko A; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia.; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Aravin AA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Esyunina D; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia., Kulbachinskiy A; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Institute of Molecular Genetics, National Research Center 'Kurchatov Institute', Moscow123182, Russia.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2023 Jun 09; Vol. 51 (10), pp. 5106-5124.