Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Lise Marie Grav"'
Autor:
Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torleif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty, Andreas H. Laustsen
Publikováno v:
mAbs, Vol 15, Iss 1 (2023)
ABSTRACTBeyond potency, a good developability profile is a key attribute of a biological drug. Selecting and screening for such attributes early in the drug development process can save resources and avoid costly late-stage failures. Here, we review
Externí odkaz:
https://doaj.org/article/40e1d35a06264ee9a178e72c2e9b24b1
Autor:
Kai Xiong, Karen Julie la Cour Karottki, Hooman Hefzi, Songyuan Li, Lise Marie Grav, Shangzhong Li, Philipp Spahn, Jae Seong Lee, Ildze Ventina, Gyun Min Lee, Nathan E. Lewis, Helene Faustrup Kildegaard, Lasse Ebdrup Pedersen
Publikováno v:
Cell Reports: Methods, Vol 1, Iss 4, Pp 100062- (2021)
Summary: Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivi
Externí odkaz:
https://doaj.org/article/becea72be57e4d009826778d02f2ad8f
Autor:
Su Hyun Kim, Seunghyeon Shin, Minhye Baek, Kai Xiong, Karen Julie la Cour Karottki, Hooman Hefzi, Lise Marie Grav, Lasse Ebdrup Pedersen, Helene Faustrup Kildegaard, Nathan E. Lewis, Jae Seong Lee, Gyun Min Lee
Chinese hamster ovary (CHO) cells are the preferred mammalian host cells for therapeutic protein production that have been extensively engineered to possess the desired attributes for high-yield protein production. However, empirical approaches for i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c0a74590e37e7af5b0db89d80c0f7979
https://doi.org/10.1101/2022.12.13.520335
https://doi.org/10.1101/2022.12.13.520335
Autor:
Tae Kwang Ha, Andreu Òdena, Karen Julie la Cour Karottki, Che Lin Kim, Hooman Hefzi, Gyun Min Lee, Helene Faustrup Kildegaard, Lars K. Nielsen, Lise Marie Grav, Nathan E. Lewis
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0f1bc0d7cf24d24464749471b5974c5
https://doi.org/10.22541/au.165606842.29880741/v1
https://doi.org/10.22541/au.165606842.29880741/v1
Autor:
Karen Julie la Cour Karottki, Songyuan Li, Shangzhong Li, Kai Xiong, Ildze Ventina, Lasse Ebdrup Pedersen, Jae Seong Lee, Nathan E. Lewis, Philipp Spahn, Hooman Hefzi, Helene Faustrup Kildegaard, Gyun Min Lee, Lise Marie Grav
Publikováno v:
Cell reports methods, vol 1, iss 4
Xiong, K, Karottki, K J L C, Hefzi, H, Li, S, Grav, L M, Li, S, Spahn, P, Lee, J S, Ventina, I, Lee, G M, Lewis, N E, Kildegaard, H F & Pedersen, L E 2021, ' An optimized genome-wide, virus-free CRISPR screen for mammalian cells ', Cell Reports Methods, vol. 1, no. 4, 100062 . https://doi.org/10.1016/j.crmeth.2021.100062
Cell reports methods
Cell Reports: Methods, Vol 1, Iss 4, Pp 100062-(2021)
Xiong, K, Karottki, K J L C, Hefzi, H, Li, S, Grav, L M, Li, S, Spahn, P, Lee, J S, Ventina, I, Lee, G M, Lewis, N E, Kildegaard, H F & Pedersen, L E 2021, ' An optimized genome-wide, virus-free CRISPR screen for mammalian cells ', Cell Reports Methods, vol. 1, no. 4, 100062 . https://doi.org/10.1016/j.crmeth.2021.100062
Cell reports methods
Cell Reports: Methods, Vol 1, Iss 4, Pp 100062-(2021)
SUMMARY Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivir
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d79b3075f3b9bb378a3fdb3111df877
https://escholarship.org/uc/item/1b08k7xg
https://escholarship.org/uc/item/1b08k7xg
Publikováno v:
Biotechnology advances. 54
Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the commercial production of therapeutic proteins. Fed-batch culture is widely used to produce therapeutic proteins, including monoclonal antibodies, because of its o
Autor:
Nathan E. Lewis, Gyun Min Lee, Philipp Spahn, Lasse Ebdrup Pedersen, Songyuan Li, Hooman Hefzi, Lise Marie Grav, Kai Xiong, Ildze Ventina, Karen Julie la Cour Karottki, Jae Seong Lee, Li Shangzhong
Publikováno v:
SSRN Electronic Journal.
Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivirus based
Publikováno v:
Sergeeva, D, Min Lee, G, Nielsen, L K & Grav, L M 2020, ' Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells ', ACS Synthetic Biology, vol. 9, no. 9, pp. 2546-2561 . https://doi.org/10.1021/acssynbio.0c00322
The ever-growing biopharmaceutical industry relies on the production of recombinant therapeutic proteins in Chinese hamster ovary (CHO) cells. The traditional timelines of CHO cell line development can be significantly shortened by the use of targete
Autor:
Philipp Spahn, Karen Julie la Cour Karottki, Shangzhong Li, Lasse Ebdrup Pedersen, Nathan E. Lewis, Hooman Hefzi, Gyun Min Lee, Kai Xiong, Songyuan Li, Helene Faustrup Kildegaard, Lise Marie Grav, Jae Seong Lee
Pooled CRISPR screens have been widely applied in mammalian cells to discover genes regulating various phenotypes of interest. In such screens, CRISPR components are generally delivered with a lentivirus. However, lentiviral CRISPR screens are limite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d364184751c32c9464509cb82d656bbf
https://doi.org/10.1101/2020.05.19.103648
https://doi.org/10.1101/2020.05.19.103648
Autor:
Helene Faustrup Kildegaard, Lise Marie Grav, Gyun Min Lee, Lasse Ebdrup Pedersen, Jae Seong Lee
Publikováno v:
Biotechnology and Bioengineering. 113:2518-2523
Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selec