Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Lisa W. Kwok"'
Autor:
Lois Pollack, Hye Yoon Park, Jonas Korlach, Lisa W. Kwok, Elizabeth Rhoades, Warren R. Zipfel, Watt W. Webb, Xiangyun Qiu
Publikováno v:
Analytical Chemistry. 78:4465-4473
We describe a microfluidic mixer that is well-suited for kinetic studies of macromolecular conformational change under a broad range of experimental conditions. The mixer exploits hydrodynamic focusing to create a thin jet containing the macromolecul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71eec50b441d0d9b3ba18e6d740d00b2
https://doi.org/10.1021/ac060572n
https://doi.org/10.1021/ac060572n
Autor:
Michael Brenowitz, Lisa W. Kwok, Hye Yoon Park, Lois Pollack, Kurt Andresen, Inna Shcherbakova, Jessica S. Lamb, Heather Smith
Publikováno v:
Journal of Molecular Biology. 355:282-293
Time-resolved small-angle X-ray scattering (SAXS) with millisecond time-resolution reveals two discrete phases of global compaction upon Mg2+-mediated folding of the Tetrahymena thermophila ribozyme. Electrostatic relaxation of the RNA occurs rapidly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3918e964ab44ff9d7b10e7c868e71064
https://doi.org/10.1016/j.jmb.2005.10.070
https://doi.org/10.1016/j.jmb.2005.10.070
Autor:
Thalia T. Mills, Sebastian Doniach, Gregory S. Maskel, Soenke Seifert, Daniel Herschlag, Simon G. J. Mochrie, Pappannan Thiyagarajan, Yu Bai, Rhiju Das, Lisa W. Kwok, Ian S. Millett, Jaby Jacob, Lois Pollack
Publikováno v:
Journal of Molecular Biology. 332:311-319
Large RNAs can collapse into compact conformations well before the stable formation of the tertiary contacts that define their final folds. This study identifies likely physical mechanisms driving these early compaction events in RNA folding. We have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc2957460fa0725fcc1ef8a99bf924f2
https://doi.org/10.1016/s0022-2836(03)00854-4
https://doi.org/10.1016/s0022-2836(03)00854-4
Autor:
Sterling Cornaby, Donald H. Bilderback, Xiangyun Qiu, Detlef-M. Smilgies, Jessica S. Lamb, Lisa W. Kwok, Kurt Andresen, Hye Yoon Park, Lois Pollack
Publikováno v:
Journal of Applied Crystallography. 40:193-195
Measurements of the global conformation of macromolecules can be carried out using small-angle X-ray scattering (SAXS). Glass focusing capillaries, manufactured at the Cornell High Energy Synchrotron Source (CHESS), have been successfully employed fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6be0db749990b476b891c9856d0e7aa2
https://doi.org/10.1107/s0021889806044505
https://doi.org/10.1107/s0021889806044505
Autor:
Jeffrey R. Krogmeier, Bryan Crane, Griffis Joshua, Lisa W. Kwok, Rudolf Gilmanshin, Nanor Kojanian, Michelle K. Nahas, Qun Zhong, Vyacheslav Papkov, Saad Shaikh, Richard Allen, Gene Malkin, Kedar Vyavahare, Robert H. Meltzer, Jonathan W. Larson, Yi Zhou, Linda Knaian
Publikováno v:
Lab on a chip. 11(5)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires differen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7225b18afc68c0c7d8513bad723047fc
https://pubmed.ncbi.nlm.nih.gov/21249264
https://pubmed.ncbi.nlm.nih.gov/21249264
Autor:
Jeffrey R. Krogmeier, Richard Allen, Qun Zhong, Yi Zhou, Griffis Joshua, Wolf Mesadieu, Kedar Vyavahare, Lisa W. Kwok, Jonathan W. Larson, Rudolf Gilmanshin, Amanda Edmonson, Linda Knaian, Bryan Crane, Robert H. Meltzer
Publikováno v:
Biophysical Journal. 96(3)
Direct Linear Analysis (DLA) technology employs continuous microfluidic elongational flow to stretch and optically map individual DNA molecules. Sequence specific tagging of DNA molecules with bisPNAs enables genomic differentiation between species.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::032545b1d788fa94374e5f6b611e7cf3
Publikováno v:
Physical Review Letters. 101
We have investigated the energetics of DNA condensation by multivalent polyamine cations. Solution small angle X-ray scattering was used to monitor interactions between short 25 base-pair dsDNA strands in the free supernatant DNA phase that coexists
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68a617190d84f488af9dddeaf474dcc2
https://doi.org/10.1103/physrevlett.101.228101
https://doi.org/10.1103/physrevlett.101.228101
Modern computing power has made it possible to reconstruct low-resolution, three-dimensional shapes from solution small-angle X-ray scattering (SAXS) data on biomolecules withouta prioriknowledge of the structure. In conjunction with rapid mixing tec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::532b082fd24c6f1dedf48514075eefd2
https://europepmc.org/articles/PMC2648657/
https://europepmc.org/articles/PMC2648657/
Autor:
Elizabeth Rhoades, M. Neal Waxham, Watt W. Webb, Jonas Korlach, Sally A. Kim, Lois Pollack, Warren R. Zipfel, Lisa W. Kwok, Hye Yoon Park
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 105(2)
A microfluidic mixer is applied to study the kinetics of calmodulin conformational changes upon Ca 2+ binding. The device facilitates rapid, uniform mixing by decoupling hydrodynamic focusing from diffusive mixing and accesses time scales of tens of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34f50b84e01bec86883aa37e97377388
https://pubmed.ncbi.nlm.nih.gov/18178620
https://pubmed.ncbi.nlm.nih.gov/18178620
Publikováno v:
Physical review letters. 99(3)
Can nonspecifically bound divalent counterions induce attraction between DNA strands? Here, we present experimental evidence demonstrating attraction between short DNA strands mediated by ${\mathrm{Mg}}^{2+}$ ions. Solution small angle x-ray scatteri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a13de51c2834843942ed104f7d7e08a
https://pubmed.ncbi.nlm.nih.gov/17678334
https://pubmed.ncbi.nlm.nih.gov/17678334