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Akademický článek

Performance of Spectrophotometric and Fluorometric DNA Quantification Methods

Autor: Brigitte Bruijns, Tina Hoekema, Lisa Oomens, Roald Tiggelaar, Han Gardeniers

Publikováno v: Analytica, Vol 3, Iss 3, Pp 371-384 (2022)

Accurate DNA quantification is a highly important method within molecular biology. Methods widely used to quantify DNA are UV spectrometry and fluorometry. In this research, seven different DNA samples and one blank (MilliQ ultrapure water) were quan

Externí odkaz: https://doaj.org/article/cead13fe7048429baf8dc29c83fce02f

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Akademický článek

Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy

Autor: Hendrika A. Segeren, Kiki C. Andree, Lisa Oomens, Bart Westendorp

Publikováno v: STAR Protocols, Vol 2, Iss 3, Pp 100718- (2021)

Summary: FACS sorting followed by single-cell RNA-sequencing (SORT-Seq) is a popular procedure to select cells of interest for single-cell transcriptomics. However, FACS is not suitable for measurement of subcellular distribution of fluorescence or f

Externí odkaz: https://doaj.org/article/146b3b1f82c1461ab207a84d7a73701f

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In Vivo Detection of Circulating Cancer-Associated Fibroblasts in Breast Tumor Mouse Xenograft: Impact of Tumor Stroma and Chemotherapy

Autor: Tao Lu, Lisa Oomens, Leon W. M. M. Terstappen, Jai Prakash

Publikováno v: Cancers, 15(4):1127. MDPI
Cancers
Volume 15
Issue 4
Pages: 1127

Cancer-associated fibroblasts (CAFs) are important drivers in the tumor microenvironment and facilitate the growth and survival of tumor cells, as well as metastasis formation. They may travel together with tumor cells to support their survival and a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12d6bc273b18d07f367fa9f5dc5ad2b2
https://doi.org/10.3390/cancers15041127

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Microsieves for the detection of circulating tumor cells in leukapheresis product in non-small cell lung cancer patients

Autor: Harry J.M. Groen, Ed Schuuring, Lisa Oomens, Leon W.M.M. Terstappen, T. Jeroen N. Hiltermann, Menno Tamminga, Arjan G.J. Tibbe, Joska Johannes Broekmaat, Kiki C. Andree

Publikováno v: Translational lung cancer research, 9(4), 1093-1100. AME PUBL CO
Translational Lung Cancer Research, 9(4), 1093-1100. Society for Translational Medicine (STM)
Transl Lung Cancer Res

Background: Circulating tumor cells (CTC) in non-small cell lung cancer (NSCLC) patients are a prognostic and possible therapeutic marker, but have a low frequency of appearance. Diagnostic leukapheresis (DLA) concentrates CTC and mononuclear cells f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81db28280c0fac7705f375823db249b9
https://doi.org/10.21037/tlcr-19-413

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Self-Seeding Microwells to Isolate and Assess the Viability of Single Circulating Tumor Cells

Autor: Lisa Oomens, Jaco Kraan, Leon W.M.M. Terstappen, Stefan Sleijfer, Joska Johannes Broekmaat, Fikri Abali, Fiona R Passanha, Pauline A J Mendelaar, Kiki C. Andree

Publikováno v: International Journal of Molecular Sciences, 20(3). Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences
Volume 20
Issue 3
International journal of molecular sciences, 20(3):477. MDPI
International Journal of Molecular Sciences, Vol 20, Iss 3, p 477 (2019)

The availability of viable tumor cells could significantly improve the disease management of cancer patients. Here we developed and evaluated a method using self-seeding microwells to obtain single circulating tumor cells (CTC) and assess their poten

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e18157d8760b9a0210c70e947063158b
https://pure.eur.nl/en/publications/c3f5daa8-3b61-4923-860e-b4f4a1247729

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VyCAP's puncher technology for single cell identification, isolation, and analysis

Autor: Fekri Abali, Joris Weersink, Lisa Oomens, Arjan G.J. Tibbe, Michiel Stevens, Joost F. Swennenhuis, Joska Johannes Broekmaat

Publikováno v: Cytometry. Part A, 93A(12), 1255-1259. Wiley-Liss Inc.

Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd16add982f0a574f11b15663fbc8034
https://research.utwente.nl/en/publications/58cef521-c7c4-4166-9c7b-76b6c3d20a4a

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