Zobrazeno 1 - 10
of 170
pro vyhledávání: '"Lisa M. Monteggia"'
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103089- (2024)
Summary: Generating stable human embryonic stem cells (hESCs) with targeted genetic mutations allows for the interrogation of protein function in numerous cellular contexts while maintaining a relatively high degree of isogenicity. We describe a step
Externí odkaz:
https://doaj.org/article/5e5744a3da6149a998a4cbad5d59afe3
Publikováno v:
Cell Reports, Vol 42, Iss 10, Pp 113201- (2023)
Summary: Calcium (Ca2+) signaling is tightly regulated within a presynaptic bouton. Here, we visualize Ca2+ signals within hippocampal presynaptic boutons using GCaMP8s tagged to synaptobrevin, a synaptic vesicle protein. We identify evoked presynapt
Externí odkaz:
https://doaj.org/article/4a7659bb7ce04609a2c6329aaacfaed8
Publikováno v:
Cell Reports, Vol 42, Iss 2, Pp 112042- (2023)
Summary: Rapid release of neurotransmitters in synchrony with action potentials is considered a key hardwired property of synapses. Here, in glutamatergic synapses formed between induced human neurons, we show that action potential-dependent neurotra
Externí odkaz:
https://doaj.org/article/fea03f4d189f4dc2a9f038f65a80da0b
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101443- (2022)
Summary: Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemi
Externí odkaz:
https://doaj.org/article/b98d09d26e5740ab817f12569d90d013
Publikováno v:
Cell Reports, Vol 37, Iss 5, Pp 109918- (2021)
Summary: Ketamine is a noncompetitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist that exerts rapid antidepressant effects. Preclinical studies identify eukaryotic elongation factor 2 kinase (eEF2K) signaling as essential for the r
Externí odkaz:
https://doaj.org/article/6dd69221221e448c8ccdfbb132cd2ff5
Publikováno v:
Cell Reports, Vol 24, Iss 6, Pp 1550-1561 (2018)
Summary: Brain-derived neurotrophic factor (BDNF) and its high-affinity receptor, tropomyosin receptor kinase B (TrkB), regulate long-term potentiation (LTP) in the hippocampus, although the sites of BDNF-TrkB receptors in this process are controvers
Externí odkaz:
https://doaj.org/article/83ee4ed526a2404bae34a2c99400a81d
Autor:
Devon C. Crawford, Denise M. O. Ramirez, Brent Trauterman, Lisa M. Monteggia, Ege T. Kavalali
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-14 (2017)
Emerging evidence suggests that spontaneous neurotransmitter release contributes to the maintenance of synaptic efficacy. Here the authors selectively reduce spontaneous glutamatergic transmission while leaving the stimulus-evoked responses intact an
Externí odkaz:
https://doaj.org/article/dc471629a1504a31be0f7fd4efcefb46
Publikováno v:
eLife, Vol 11 (2022)
Synapses maintain both action potential-evoked and spontaneous neurotransmitter release; however, organization of these two forms of release within an individual synapse remains unclear. Here, we used photobleaching properties of iGluSnFR, a fluoresc
Externí odkaz:
https://doaj.org/article/ba5f7accb51f4f63b4ac60f611f5e381
Publikováno v:
Trends in Molecular Medicine. 29:364-375
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4caa2ccfad67690854621fa851619c68
https://doi.org/10.1016/j.molmed.2023.02.003
https://doi.org/10.1016/j.molmed.2023.02.003
Publikováno v:
eLife, Vol 9 (2020)
Synaptic transmission is initiated via spontaneous or action-potential evoked fusion of synaptic vesicles. At excitatory synapses, glutamatergic receptors activated by spontaneous and evoked neurotransmission are segregated. Although inhibitory synap
Externí odkaz:
https://doaj.org/article/5d42b65ad1034437a3ace174f99c440f