LAMP4yaws: Treponema pallidum, Haemophilus ducreyi loop mediated isothermal amplification − protocol for a cross-sectional, observational, diagnostic accuracy study

Autor: Michael Marks, Tania Crucitti, Emma Michele Harding-Esch, Daniel Arhinful, Earnest Njih Tabah, Serges Tchatchouang, Oriol Mitja, Becca Louise Handley, Camila González-Beiras, Laud Antony Basing, Kouadio Aboh Hugues, Mohammed Bakheit, Lisa Becherer, Christina Ries, Nadine Borst, Simone Lüert, Sieghard Frischmann, Tamara Haerpfer, Emelie Landmann, Ivy Amanor, Aboubacar Sylla, Mireille S Kouamé-Sina, Jean P Ndzomo-Ngono, Adingra Tano, Patrick Awondo, Solange Ngazoa Kakou, Sara Eyangoh, Kennedy Kwasi Addo, Sascha Knauf

Publikováno v: BMJ Open, Vol 12, Iss 3 (2022)

Introduction Yaws, caused by the bacterium Treponema pallidum subsp. pertenue, is a neglected tropical disease targeted for eradication by 2030. Improved diagnostics will be essential to meet this goal. Diagnosis of yaws has relied heavily on clinica

Externí odkaz: https://doaj.org/article/61bbd23db90d43ea9fe47ffb71d78869

Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP

Autor: Lisa Becherer, Jacob Friedrich Hess, Sieghard Frischmann, Mohammed Bakheit, Hans Nitschko, Silvina Stinco, Friedrich Zitz, Hannes Hofer, Giampiero Porro, Florian Hausladen, Karl Stock, Dominik Drossart, Holger Wurm, Hanna Kuhn, Dominik Huber, Tobias Hutzenlaub, Nils Paust, Mark Keller, Oliver Strohmeier, Simon Wadle, Nadine Borst, Roland Zengerle, Felix von Stetten

Publikováno v: Micromachines, Vol 12, Iss 2, p 159 (2021)

This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD

Externí odkaz: https://doaj.org/article/4a5b12acd76d4d2a9e78791e90dfe337

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Autor: Nadine Borst, Lisa Becherer, Roland Zengerle, Sieghard Frischmann, Felix von Stetten, Mohammed A. Bakheit

Publikováno v: Analytical Methods. 12:717-746

In the course of the last 20 years, isothermal nucleic acid amplification tests have emerged as an important diagnostic tool, not only for clinical applications, but also for food quality control and environmental monitoring. Loop-mediated isothermal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::97c0355c0e828b3c127ad0a097ccbaa0
https://doi.org/10.1039/c9ay02246e

Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi

Autor: Nadine Borst, Sieghard Frischmann, Sibauk Bieb, Kingsley Asiedu, Yaw Adu-Sarkodie, Oriol Mitjà, Sascha Knauf, Lisa Becherer, Felix von Stetten, Michael Marks, Mohammed A. Bakheit, Simone Lueert

Publikováno v: Emerging Infectious Diseases, Vol 26, Iss 2, Pp 282-288 (2020)
Emerging Infectious Diseases
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
instname

Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to dist

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0880ce9c52ece47dfae4e13a7a386f7f

A technology platform for digital nucleic acid diagnostics at the point of care

Autor: Ana Belén Rodríguez-Martínez, Simon Wadle, Lisa Becherer, Jia Li, Mara Specht, Martin Trotter, Friedrich Schuler, Martin Schulz, Roland Zengerle, Nadine Borst, Felix von Stetten, Nils Paust

Publikováno v: LaboratoriumsMedizin. 41:245-249

The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Diffe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6bb7500ea43dd754e7e14dc4f7cad3eb
https://doi.org/10.1515/labmed-2017-0098