An entirely cell-based system to generate single-chain antibodies against cell surface receptors.

Autor: Lipes, BD, Chen, YH, Ma, H, Staats, HF, Kenan, DJ, Gunn, MD

The generation of recombinant antibodies (Abs) using phage display is a proven method to obtain a large variety of Abs that bind with high affinity to a given antigen. Traditionally, the generation of single-chain Abs depends on the use of recombinan

Externí odkaz: http://www.ncbi.nlm.nih.gov/pubmed/18455737
http://hdl.handle.net/10161/903

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Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR.

Autor: Fontes CM; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Lipes BD; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA., Liu J; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Agans KN; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.; Galveston National Laboratory, University of Texas Medical Branch at Galveston, Galveston, TX 77550, USA., Yan A; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA., Shi P; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA., Cruz DF; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Kelly G; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Luginbuhl KM; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Joh DY; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Foster SL; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.; Galveston National Laboratory, University of Texas Medical Branch at Galveston, Galveston, TX 77550, USA., Heggestad J; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Hucknall A; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Mikkelsen MH; Department of Electrical and Computer Engineering, Duke University, Durham, NC 27708, USA., Pieper CF; Departments of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27710, USA., Horstmeyer RW; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Geisbert TW; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.; Galveston National Laboratory, University of Texas Medical Branch at Galveston, Galveston, TX 77550, USA., Gunn MD; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. michael.gunn@duke.edu ashutosh.chilkoti@duke.edu., Chilkoti A; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. michael.gunn@duke.edu ashutosh.chilkoti@duke.edu.

Publikováno v: Science translational medicine [Sci Transl Med] 2021 Apr 07; Vol. 13 (588).

An entirely cell-based system to generate single-chain antibodies against cell surface receptors.

Autor: Lipes BD; Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA., Chen YH, Ma H, Staats HF, Kenan DJ, Gunn MD

Publikováno v: Journal of molecular biology [J Mol Biol] 2008 May 30; Vol. 379 (2), pp. 261-72. Date of Electronic Publication: 2008 Apr 07.

Pulsed-field gel electrophoresis.

Autor: Wrestler JC; Department of Pharmacology, University of North Carolina, Chapel Hill 27599, USA., Lipes BD, Birren BW, Lai E

Publikováno v: Methods in enzymology [Methods Enzymol] 1996; Vol. 270, pp. 255-72.