Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Line Dahl Poulsen"'
Publikováno v:
BioTechniques, Vol 67, Iss 3, Pp 110-116 (2019)
RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3′ ends of cDNAs often produces primer–adapter byproducts, which compete with cDNA–adapter ligation products during library amplification and, therefore, red
Externí odkaz:
https://doaj.org/article/707445135cae488187e1dc2266a6113a
Autor:
Adrian Sven Geissler, Annaleigh Ohrt Fehler, Line Dahl Poulsen, Enrique González-Tortuero, Thomas Beuchert Kallehauge, Ferhat Alkan, Christian Anthon, Stefan Ernst Seemann, Michael Dolberg Rasmussen, Anne Breüner, Carsten Hjort, Jeppe Vinther, Jan Gorodkin
Publikováno v:
Geissler, A S, Fehler, A O, Poulsen, L D, Gonzalez-Tortuero, E, Kallehauge, T B, Alkan, F, Anthon, C, Seemann, S E, Rasmussen, M D, Breuener, A, Hjort, C, Vinther, J & Gorodkin, J 2023, ' CRISPRi screen for enhancing heterologous alpha-amylase yield in Bacillus subtilis ', Journal of Industrial Microbiology and Biotechnology, vol. 50, no. 1, kuac028 . https://doi.org/10.1093/jimb/kuac028
Yield improvements in cell factories can potentially be obtained by fine-tuning the regulatory mechanisms for gene candidates. In pursuit of such candidates, we performed RNA-sequencing of two α-amylase producing Bacillus strains and predict hundred
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8ab4bc4d4013dd5d0ff231de64602d9
https://doi.org/10.1093/jimb/kuac028
https://doi.org/10.1093/jimb/kuac028
Autor:
Dominika Jedrzejczyk, Line Dahl Poulsen, Marina Mohr, Nkerorema Djodji Damas, Sanne Schoffelen, Andrea Barghetti, Roland Baumgartner, Brian Tate Weinert, Tanya Warnecke, Ryan Gill
CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts. As opposed to Cas9, the Cas12a endonucleases are characterized by a lack of tran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::75a97f3b34a81b4f8c2760c120f5625b
https://doi.org/10.21203/rs.3.rs-1003466/v1
https://doi.org/10.21203/rs.3.rs-1003466/v1
Autor:
Adrian Sven, Geissler, Christian, Anthon, Ferhat, Alkan, Enrique, González-Tortuero, Line Dahl, Poulsen, Thomas Beuchert, Kallehauge, Anne, Breüner, Stefan Ernst, Seemann, Jeppe, Vinther, Jan, Gorodkin
Publikováno v:
Microbial Genomics
A large part of our current understanding of gene regulation in Gram-positive bacteria is based on Bacillus subtilis , as it is one of the most well studied bacterial model systems. The rapid growth in data concerning its molecular and genomic biolog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::98caddc191271fc7b28d6c2e53c4ea53
https://pubmed.ncbi.nlm.nih.gov/33539279
https://pubmed.ncbi.nlm.nih.gov/33539279
Autor:
Jan Gorodkin, Christian Anthon, Enrique González-Tortuero, Ferhat Alkan, Adrian Sven Geissler, Thomas Beuchert Kallehauge, Jeppe Vinther, Line Dahl Poulsen, Stefan E. Seemann, Anne Breüner
Publikováno v:
Geissler, A S, Anthon, C, Alkan, F, González-Tortuero, E, Poulsen, L D, Kallehauge, T B, Breüner, A, Seemann, S E, Vinther, J & Gorodkin, J 2021, ' Bsgatlas : A unified bacillus subtilis genome and transcriptome annotation atlas with enhanced information access ', Microbial Genomics, vol. 7, no. 2, 000524 . https://doi.org/10.1099/mgen.0.000524
A large part of our current understanding of gene regulation in Gram-positive bacteria is based on Bacillus subtilis , as it is one of the most well studied bacterial model systems. The rapid growth in data concerning its molecular and genomic biolog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96ba393080c704963aae85cc45b6724f
https://curis.ku.dk/portal/da/publications/bsgatlas(b3ab8dd5-f20e-4158-a71b-617bf43751fc).html
https://curis.ku.dk/portal/da/publications/bsgatlas(b3ab8dd5-f20e-4158-a71b-617bf43751fc).html
Autor:
Finn Kirpekar, Anders Albrechtsen, Line Dahl Poulsen, Christel Enroth, Jeppe Vinther, Søren Iversen
Publikováno v:
Enroth, C, Poulsen, L D, Iversen, S, Kirpekar, F, Albrechtsen, A & Vinther, J 2019, ' Detection of internal N7-methylguanosine (m7G) RNA modifications by mutational profiling sequencing ', Nucleic acids research, vol. 47, no. 20, e126 . https://doi.org/10.1093/nar/gkz736
Nucleic Acids Research
Enroth, C, Poulsen, L D, Iversen, S, Kirpekar, F, Albrechtsen, A & Vinther, J 2019, ' Detection of internal N7-methylguanosine (m 7 G) RNA modifications by mutational profiling sequencing ', Nucleic Acids Research, vol. 47, no. 20, e126 . https://doi.org/10.1093/nar/gkz736
Nucleic Acids Research
Enroth, C, Poulsen, L D, Iversen, S, Kirpekar, F, Albrechtsen, A & Vinther, J 2019, ' Detection of internal N7-methylguanosine (m 7 G) RNA modifications by mutational profiling sequencing ', Nucleic Acids Research, vol. 47, no. 20, e126 . https://doi.org/10.1093/nar/gkz736
Methylation of guanosine on position N7 (m7G) on internal RNA positions has been found in all domains of life and have been implicated in human disease. Here, we present m7G Mutational Profiling sequencing (m7G-MaP-seq), which allows high throughput
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad865d1de5bdcf043ec06b49d74dc68c
https://findresearcher.sdu.dk:8443/ws/files/157065331/gkz736.pdf
https://findresearcher.sdu.dk:8443/ws/files/157065331/gkz736.pdf
Autor:
Jan Gorodkin, Enrique González-Tortuero, Jeppe Vinther, Thomas Beuchert Kallehauge, Stefan E. Seemann, Line Dahl Poulsen, Christian Anthon, Adrian Sven Geissler
The genome of Bacillus subtilis continues to provide exiting genomic insights. However, the growing collective genomic knowledge about this micro-organism is spread across multiple annotation resources. Thus, the full annotation is not directly acces
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e05f434a331eae921a3130834088e28d
Publikováno v:
Poulsen, L D, Kielpinski, L J, Salama, S R, Krogh, A & Vinther, J 2015, ' SHAPE selection (SHAPES) enrich for RNA structure signal in SHAPE sequencing-based probing data ', R N A, vol. 21, no. 5, pp. 1042-1052 . https://doi.org/10.1261/rna.047068.114
Selective 2′ Hydroxyl Acylation analyzed by Primer Extension (SHAPE) is an accurate method for probing of RNA secondary structure. In existing SHAPE methods, the SHAPE probing signal is normalized to a no-reagent control to correct for the backgrou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91815aba07cb253ffff5d630c125e613
https://doi.org/10.1261/rna.047068.114
https://doi.org/10.1261/rna.047068.114
Autor:
Line Dahl Poulsen, Jeppe Vinther
Publikováno v:
Poulsen, L D & Vinther, J 2018, ' RNA-Seq for Bacterial Gene Expression ', Current Protocols in Nucleic Acid Chemistry, vol. 73, no. 1, e55, pp. 1-12 . https://doi.org/10.1002/cpnc.55
RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1c4195bc2bf994a06aa7244c42847cf
https://curis.ku.dk/portal/da/publications/rnaseq-for-bacterial-gene-expression(bca1eef6-144c-4bf7-94e7-3784453f0b07).html
https://curis.ku.dk/portal/da/publications/rnaseq-for-bacterial-gene-expression(bca1eef6-144c-4bf7-94e7-3784453f0b07).html