Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Linda G. Lee"'
Publikováno v:
Biosensors, Vol 3, Iss 4, Pp 360-373 (2013)
We demonstrate a fluorescence lateral flow system that has excellent sensitivity and wide dynamic range. The illumination system utilizes an LED, plastic lenses and plastic and colored glass filters for the excitation and emission light. Images are c
Externí odkaz:
https://doaj.org/article/35af2b5881694824bd775b536544b48b
Publikováno v:
Biosensors, Vol 3, Iss 4, Pp 360-373 (2013)
Biosensors
Biosensors; Volume 3; Issue 4; Pages: 360-373
Biosensors
Biosensors; Volume 3; Issue 4; Pages: 360-373
We demonstrate a fluorescence lateral flow system that has excellent sensitivity and wide dynamic range. The illumination system utilizes an LED, plastic lenses and plastic and colored glass filters for the excitation and emission light. Images are c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d1d56f098b6ece890aeb6625ac12a19
https://doi.org/10.3390/bios3040360
https://doi.org/10.3390/bios3040360
Publikováno v:
Cytometry. 21:120-128
Several fluorescent dyes that absorb in the nearinfrared are described. The photostability and aggregation properties of the dyes were examined. Two of the dyes, BHMP and BHDMAP, emit at 805 nm and were useful dyes for protein labeling. A dual-laser,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::441b8ac6d514476df3e2bc72d5d96562
https://doi.org/10.1002/cyto.990210203
https://doi.org/10.1002/cyto.990210203
Autor:
Ronald J. Graham, Linda G. Lee, Sonia S. Connaughton, Karen E. Low, Melissa Gee, Hongye Sun, Richard L. Noble, Sam L. Woo
Publikováno v:
Analytical chemistry. 77(7)
We report a novel, real-time fluorogenic kinase assay. The peptide substrates are synthesized with a fluorescent dye and a hydrocarbon tail. The substrate self-assembles into micelles, increasing the local concentration of the dye and quenching its f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42a935bb1a3b99c79145ff6893d16a63
https://pubmed.ncbi.nlm.nih.gov/15801736
https://pubmed.ncbi.nlm.nih.gov/15801736
Autor:
S. L. Spurgeon, Linda G. Lee, Steven M. Menchen, S. M. Chen, C. R. Heiner, Barnett B. Rosenblum, Shaheer H. Khan
Publikováno v:
Nucleic acids research. 25(22)
We have used two new dye sets for automated dye-labeled terminator DNA sequencing. One set consists of four, 4,7-dichlororhodamine dyes (d-rhodamines). The second set consists of energy-transfer dyes that use the 5-carboxy-d-rhodamine dyes as accepto
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29ee3b207a72e11c296ef666fc38ff0f
https://pubmed.ncbi.nlm.nih.gov/9358158
https://pubmed.ncbi.nlm.nih.gov/9358158
Autor:
A. Constantinescu, Krishna G. Upadhya, Linda G. Lee, Ronald J. Graham, Scott C. Benson, Barnett B. Rosenblum, Jonathan M. Cassel, Sandra L. Spurgeon, C. R. Heiner, Steven M. Menchen
Publikováno v:
Nucleic acids research. 25(14)
We have synthesized a set of four energy transfer dyes and demonstrated their use in automated DNA sequencing. The donor dyes are the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein and the acceptor dyes are a novel set of four 4,7-dichloro-subs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d800854ca46e9b27c9ab5c585b56e6e
https://pubmed.ncbi.nlm.nih.gov/9207029
https://pubmed.ncbi.nlm.nih.gov/9207029
Publikováno v:
Nucleic acids research. 21(16)
Nick-translation PCR was performed with fluorogenic probes. Two probes were used: one complementary to a sequence containing the F508 codon of the normal human cystic fibrosis (CF) gene (wt DNA) and one complementary to a sequence containing the delt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::184ba42bb9ed60a754217a6aae9596bd
https://pubmed.ncbi.nlm.nih.gov/8367293
https://pubmed.ncbi.nlm.nih.gov/8367293
Autor:
Charles R. Connell, Nicolette D. Halloran, Richard D. Cheng, Linda G. Lee, Carl W. Fuller, Rick K. Wilson, Sam Lee Woo, Bernard F. McArdle
Publikováno v:
Nucleic acids research. 20(10)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f12e20e63358d39b38938e637ac04127
https://pubmed.ncbi.nlm.nih.gov/1598205
https://pubmed.ncbi.nlm.nih.gov/1598205
Publikováno v:
Cytometry. 10:151-164
Several new derivatives of fluorescein were synthesized. The dyes were characterized by NMR; and the absorbance, excitation, and emission spectra were measured. The fluorescence quantum yields of the dyes were determined. The pKa3 values of the dyes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121c2161d8f6bce74ea1eea27ecf20b6
https://doi.org/10.1002/cyto.990100206
https://doi.org/10.1002/cyto.990100206
Publikováno v:
Cytometry. 7:508-517
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d38958c5e9e7687dac271120e8ca82f5
https://doi.org/10.1002/cyto.990070603
https://doi.org/10.1002/cyto.990070603