Quantitative Real-Time PCR Based on SYBR Green Technology for the Identification of Philaenus italosignus Drosopoulos & Remane (Hemiptera Aphrophoridae)

Autor: Domenico Rizzo, Matteo Bracalini, Sara Campigli, Anita Nencioni, Francesco Porcelli, Guido Marchi, Daniele Da Lio, Linda Bartolini, Elisabetta Rossi, Patrizia Sacchetti, Tiziana Panzavolta

Publikováno v: Plants, Vol 11, Iss 23, p 3314 (2022)

The use of molecular tools to identify insect pests is a critical issue, especially when rapid and reliable tests are required. We proposed a protocol based on qPCR with SYBR Green technology to identify Philaenus italosignus (Hemiptera, Aphrophorida

Externí odkaz: https://doaj.org/article/cd88ef760cb247c38c19840bf7e2e462

Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection of Geosmithia morbida (Kolařik) in Infected Walnut

Autor: Domenico Rizzo, Chiara Aglietti, Alessandra Benigno, Matteo Bracalini, Daniele Da Lio, Linda Bartolini, Giovanni Cappellini, Antonio Aronadio, Cristina Francia, Nicola Luchi, Alberto Santini, Santa Olga Cacciola, Tiziana Panzavolta, Salvatore Moricca

Publikováno v: Plants, Vol 11, Iss 9, p 1239 (2022)

Walnut species (Juglans spp.) are multipurpose trees, widely employed in plantation forestry for high-quality timber and nut production, as well as in urban greening as ornamental plants. These species are currently threatened by the thousand cankers

Externí odkaz: https://doaj.org/article/5966435765704d929f8175b9e2544a25

SYBR Green‐based real‐time PCR test for the identification of adults and larvae of the Japanese beetle Popillia japonica Newman (Coleoptera Scarabaeidae)

Autor: Domenico Rizzo, Daniele Da Lio, Tommaso Bruscoli, Giovanni Cappellini, Linda Bartolini, Chiara Salemi, Antonio Aronadio, Dalia Del Nista, Jason B. Oliver, Elisabetta Rossi

Publikováno v: EPPO Bulletin. 52:141-148

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3fee65382ca5d03db8e97db01248f898
https://doi.org/10.1111/epp.12839

DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues

Autor: Domenico Rizzo, Daniele Da Lio, Linda Bartolini, Cristina Francia, Antonio Aronadio, Nicola Luchi, Sara Campigli, Guido Marchi, Elisabetta Rossi

Publikováno v: Plant Pathology ISBN: 9781071625163

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71bed62a12415f7e698add8c0da6b6e8
https://doi.org/10.1007/978-1-0716-2517-0_5

Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Point and RT-qPCR Methods

Autor: Giuseppe Parrella, Daniele Da Lio, Giovanni Cappellini, Linda Bartolini, Chiara Salemi, Alessandra Panattoni, Domenico Rizzo

Publikováno v: Frontiers in Microbiology
Frontiers in microbiology (2021). doi:10.3389/fmicb.2021.640932
info:cnr-pdr/source/autori:Rizzo D., Da Lio D., Panattoni A., Salemi C., Cappellini G., Bartolini L., Parrella G./titolo:Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Point and RT-qPCR Methods/doi:10.3389%2Ffmicb.2021.640932/rivista:Frontiers in microbiology/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume
Frontiers in Microbiology, Vol 12 (2021)

Tomato brown rugose fruit virus (ToBRFV) represents an emerging viral threat to the productivity of tomato and pepper protected cultivation worldwide. This virus has got the status of quarantine organism in the European Union (EU) countries. In parti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08af95a77a3cf2c786c27aab970ba1d3
http://europepmc.org/articles/PMC8096992