Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Lin Tang, Goh"'
Autor:
Ryan Haryadi, Steven Ho, Yee Jiun Kok, Helen X Pu, Lu Zheng, Natasha A Pereira, Bin Li, Xuezhi Bi, Lin-Tang Goh, Yuansheng Yang, Zhiwei Song
Publikováno v:
PLoS ONE, Vol 10, Iss 2, p e0116878 (2015)
Translocation of a nascent protein from the cytosol into the ER mediated by its signal peptide is a critical step in protein secretion. The aim of this work was to develop a platform technology to optimize the signal peptides for high level productio
Externí odkaz:
https://doaj.org/article/39d0379794ba40608a271e6f893ea807
Autor:
Steven C L Ho, Muriel Bardor, Bin Li, Jia Juan Lee, Zhiwei Song, Yen Wah Tong, Lin-Tang Goh, Yuansheng Yang
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e63247 (2013)
Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on
Externí odkaz:
https://doaj.org/article/061242949654436eaaecd8c51b9d3a9f
Autor:
Say Kong Ng, Tessa Rui Min Tan, Yang Wang, Daniel Ng, Lin-Tang Goh, Muriel Bardor, Victor Vai Tak Wong, Kong Peng Lam
Publikováno v:
PLoS ONE, Vol 7, Iss 12, p e52785 (2012)
Dectin-1 (CLEC7A) is a C-type lectin receptor that binds to β-glucans found in fungal cell walls to act as a major pattern recognition receptor (PRR). Since β-glucans epitope is not present in human cells, we are of the opinion that Dectin-1 can ha
Externí odkaz:
https://doaj.org/article/9a6db7d4973f4f33a9bef705f57e9b5e
Publikováno v:
Journal of Proteome Research. 12:3496-3510
Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7f95c203c237ec2ec80c28d2309fac4
https://doi.org/10.1021/pr400352n
https://doi.org/10.1021/pr400352n
Autor:
Satty G. Reddy, Lin Tang Goh, Chew-Kiat Heng, Ying Swan Ho, William P. K. Chong, Miranda G.S. Yap, Niki S.C. Wong, Faraaz N.K. Yusufi, Dong-Yup Lee
Publikováno v:
Rapid Communications in Mass Spectrometry. 23:3763-3771
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium was collected and analysed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b16b5ab8504b277c4719f33845b4d028
https://doi.org/10.1002/rcm.4328
https://doi.org/10.1002/rcm.4328
Publikováno v:
Biotechnology and Bioengineering. 95:946-960
Glycosylation has profound effects on the quality of recombinant proteins produced in mammalian cells. The biosynthetic pathways of N-linked glycans on glycoproteins involves a relatively small number of enzymes and nucleotide sugars. Many of these g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49e311265fe7b7431502e29db81a7b0e
https://doi.org/10.1002/bit.21062
https://doi.org/10.1002/bit.21062
Publikováno v:
Biotechnology and Bioengineering. 95:911-918
Monoclonal antibody therapeutics is an important and fast expanding market. While production of these molecules has been a major area of research, much less is known regarding the stabilization of these proteins for delivery as drugs. Crystallization
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81b01f303bee770afab6a8839012779b
https://doi.org/10.1002/bit.21054
https://doi.org/10.1002/bit.21054
Autor:
Miranda Gek Sim Yap, Lin-Tang Goh
Publikováno v:
Journal of Separation Science. 28:2104-2110
A robust tandem HPLC method coupling size-exclusion (Shodex Asahipak GS-320HQ) and reversed phase (Vydac 218TP54) columns with ultraviolet detection was developed for quantitative determination of interferon-gamma (IFN-gamma) in Chinese hamster ovary
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb48ede659acc3fb4166bfb6ae15ffeb
https://doi.org/10.1002/jssc.200500045
https://doi.org/10.1002/jssc.200500045
Publikováno v:
Biotechnology and Bioengineering. 89:164-177
As we pursue the means to improve yields to meet growing therapy demands, it is important to examine the impact of process control on glycosylation patterns to ensure product efficacy and consistency. In this study, we describe a dynamic on-line fed-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48f313e4d28b0b7a54e24ea337ef684f
https://doi.org/10.1002/bit.20317
https://doi.org/10.1002/bit.20317
Publikováno v:
Biotechnology Letters. 23:661-665
The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and expressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a fed-batch bioreactor feeding first glycerol then followed by methanol. Recombinant Blo t 5 constitute
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b05d2891ef697d306f2efdf0be26f582
https://doi.org/10.1023/a:1010323611039
https://doi.org/10.1023/a:1010323611039