Zobrazeno 1 - 10
of 1 375
pro vyhledávání: '"Library preparation"'
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-25 (2024)
Abstract Whole genome sequencing (WGS) has become a gold standard for diagnosing genomic variation. Peripheral blood is a common sample source for the extraction of nucleic acids for Next-Generation Sequencing (NGS) applications. Here, we present an
Externí odkaz:
https://doaj.org/article/c4c5450e3cb9491986eb70f5d59d30e1
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024)
Abstract Transposon directed insertion-site sequencing (TraDIS), a variant of transposon insertion sequencing commonly known as Tn-Seq, is a high-throughput assay that defines essential bacterial genes across diverse growth conditions. However, the v
Externí odkaz:
https://doaj.org/article/c659783f99e34eb3abf53f3d762404e0
Autor:
Elody Orcel, Hayat Hage, May Taha, Noémie Boucher, Emilie Chautard, Virginie Courtois, Adrien Saliou
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-16 (2024)
Abstract Background Blood transcriptomic analysis is widely used to provide a detailed picture of a physiological state with potential outcomes for applications in diagnostics and monitoring of the immune response to vaccines. However, multi-species
Externí odkaz:
https://doaj.org/article/d5c4a753fb8249b19f6ee4c60a79d693
Publikováno v:
Frontiers in Oncology, Vol 14 (2024)
In clinical oncology, cell-free DNA (cfDNA) has shown immense potential in its ability to noninvasively detect cancer at various stages and monitor the progression of therapy. Despite the rapid improvements in cfDNA liquid biopsy approaches, achievin
Externí odkaz:
https://doaj.org/article/604d9dcc762f4209820eacf7a6ee1400
Publikováno v:
BMC Research Notes, Vol 16, Iss 1, Pp 1-7 (2023)
Abstract Objective Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection
Externí odkaz:
https://doaj.org/article/21918d562f1642a4a31fe4e091944c63
Autor:
Alexander Hesselberg Løvestad, Milan S. Stosic, Jean-Marc Costanzi, Irene Kraus Christiansen, Hege Vangstein Aamot, Ole Herman Ambur, Trine B. Rounge
Publikováno v:
Virology Journal, Vol 20, Iss 1, Pp 1-11 (2023)
Abstract Background Previously developed TaME-seq method for deep sequencing of HPV, allowed simultaneous identification of the human papillomavirus (HPV) DNA consensus sequence, low-frequency variable sites, and chromosomal integration events. The m
Externí odkaz:
https://doaj.org/article/18b69713c59d42ed8f870dff46754b8a
Autor:
Benjamin M. David, Paul A. Jensen
Publikováno v:
SLAS Technology, Vol 28, Iss 1, Pp 16-21 (2023)
In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so abundant that small improvements in depletion efficiency lead to large increases in mRNA sequencing coverage.
Externí odkaz:
https://doaj.org/article/802e92bdcbbd4b8682f067ee3b0bbf3c
Autor:
Žana Kapustina, Justina Medžiūnė, Varvara Dubovskaja, Karolis Matjošaitis, Simona Žeimytė, Arvydas Lubys
Publikováno v:
RNA Biology, Vol 19, Iss 1, Pp 774-780 (2022)
High-throughput RNA sequencing offers a comprehensive analysis of transcriptome complexity originated from regulatory events, such as differential gene expression, alternative polyadenylation and others, and allows the increase in diagnostic capacity
Externí odkaz:
https://doaj.org/article/f318a9d4163a445b87f5f7d5ae7267c9
Autor:
Jieyu Zhao, Eugene Yui-Ching Chow, Pui Yan Yeung, Qiangfeng Cliff Zhang, Ting-Fung Chan, Chun Kit Kwok
Publikováno v:
BMC Biology, Vol 20, Iss 1, Pp 1-19 (2022)
Abstract Background RNA G-quadruplexes (rG4s) are non-canonical structural motifs that have diverse functional and regulatory roles, for instance in transcription termination, alternative splicing, mRNA localization and stabilization, and translation
Externí odkaz:
https://doaj.org/article/f5f103a94e2c4e01ab2965063885bbfa
Publikováno v:
BMC Biology, Vol 20, Iss 1, Pp 1-12 (2022)
Abstract Background Shotgun metagenomic sequencing has greatly expanded the understanding of microbial communities in various biological niches. However, it is still challenging to efficiently convert sub-nanogram DNA to high-quality metagenomic libr
Externí odkaz:
https://doaj.org/article/9d25a34a42074f808aeda8d6d2f9db69