Immunoaffinity pre-concentration combined with on-column visual detection as a tool for rapid aflatoxin M1 screening in milk

Autor: Makpal A. Karagusheva, Carlos Van Peteghem, Sarah De Saeger, Liberty Sibanda, Irina Yu. Goryacheva

Publikováno v: Food Control. 20:802-806

A non-instrumental rapid test was developed for the screening of aflatoxin M1 (AfM1) in milk at the 40 ng/L level. The method combines on one immunoaffinity gel layer, AfM1 pre-concentration and direct competitive immunoassay detection with visual ev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::89054e541a740803f3e3feca1b55000e
https://doi.org/10.1016/j.foodcont.2008.10.011

Application and validation of a clean-up tandem assay column for screening ochratoxin A in cocoa powder

Autor: Liberty Sibanda, Marieke Lobeau, I Barna-Vetro, Carlos Van Peteghem, Sarah De Saeger

Publikováno v: Food Additives and Contaminants
Food Additives and Contaminants, 2007, 24 (04), pp.398-405. ⟨10.1080/02652030601053162⟩

A rapid antibody-based assay for the detection of ochratoxin A in cocoa powder is described, involving sequential clean-up and visual detection of the toxin ("clean-up tandem assay column"). The screening test was developed to have a cut-off level of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a51625c22181a2e9ddde051e1e7c0be8
https://doi.org/10.1080/02652030601053162

Development of a new clean-up tandem assay column for the detection of ochratoxin A in roasted coffee

Autor: Marieke Lobeau, Liberty Sibanda, I Barna-Vetro, C. Van Peteghem, S. De Saeger

Publikováno v: Analytica Chimica Acta. 538:57-61

As the mycotoxin ochratoxin A (OTA) can occur in roasted coffee, regulations and maximum limits are necessary. The need for simple, rapid and inexpensive detection methods which require a minimum of equipment led to the development of a clean-up tand

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7850c1ace72e71b7c23e044c04691406
https://doi.org/10.1016/j.aca.2005.02.010

A flow-through enzyme immunoassay for the screening of fumonisins in maize

Autor: S. De Saeger, Liberty Sibanda, C. Van Peteghem, I. Léglise, F. Van Hove, I Barna-Vetro, C. Paepens

Publikováno v: Analytica Chimica Acta. 523:229-235

The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B-1 (FB1) and B-2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::12cc2cb4915ecc579ae93bece1169dc7
https://doi.org/10.1016/j.aca.2004.07.044

Development of a Solid-Phase Cleanup and Portable Rapid Flow-Through Enzyme Immunoassay for the Detection of Ochratoxin A in Roasted Coffee

Autor: Sarah De Saeger, Ildiko Barna-Vetro, Carlos Van Peteghem, Liberty Sibanda

Publikováno v: Journal of Agricultural and Food Chemistry. 50:6964-6967

A membrane-based flow-through enzyme immunoassay (patent application pending) for the detection of ochratoxin A (OA) in roasted coffee was developed. First, an extraction and solid-phase cleanup method was developed. A high partition coefficient for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::681d55cec06f54cb07ca323cf919b312
https://doi.org/10.1021/jf020553o