Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

Autor: Yaron TM; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; Tri-Institutional PhD Program in Computational Biology and Medicine, Weill Cornell Medicine/Memorial Sloan Kettering Cancer Center/Rockefeller University, New York, NY 10021, USA., Heaton BE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Levy TM; Cell Signaling Technology, Danvers, MA 01923, USA., Johnson JL; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Jordan TX; Grossman School of Medicine, New York University, New York, NY 10016, USA., Cohen BM; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Kerelsky A; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA., Lin TY; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.; Cell and Developmental Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA., Liberatore KM; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Bulaon DK; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA., Van Nest SJ; Department of Radiation Oncology, Weill Cornell Medicine, New York, NY 10065, USA., Koundouros N; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Pharmacology, Weill Cornell Medicine, New York, NY 10065, USA., Kastenhuber ER; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Mercadante MN; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Shobana-Ganesh K; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.; Cell and Developmental Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA., He L; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA., Schwartz RE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; Division of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Chen S; Department of Surgery, Weill Cornell Medicine, New York, NY 10065, USA., Weinstein H; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA., Elemento O; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA., Piskounova E; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Dermatology, Weill Cornell Medicine, New York, NY 10065, USA., Nilsson-Payant BE; Grossman School of Medicine, New York University, New York, NY 10016, USA., Lee G; Department of Microbiology and Molecular Genetics, Chao Family Comprehensive Cancer Center, University of California, Irvine School of Medicine, Irvine, CA 92868, USA., Trimarco JD; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Burke KN; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Hamele CE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Chaparian RR; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Harding AT; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Tata A; Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA., Zhu X; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Tata PR; Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USA., Smith CM; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA., Possemato AP; Cell Signaling Technology, Danvers, MA 01923, USA., Tkachev SL; Cell Signaling Technology, Danvers, MA 01923, USA., Hornbeck PV; Cell Signaling Technology, Danvers, MA 01923, USA., Beausoleil SA; Cell Signaling Technology, Danvers, MA 01923, USA., Anand SK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Aguet F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Getz G; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.; Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.; Cancer Center and Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA., Davidson AD; School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK., Heesom K; Proteomics Facility, University of Bristol, Bristol BS8 1TD, UK., Kavanagh-Williamson M; School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK., Matthews DA; School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK., tenOever BR; Grossman School of Medicine, New York University, New York, NY 10016, USA., Cantley LC; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.; Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA., Blenis J; Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Pharmacology, Weill Cornell Medicine, New York, NY 10065, USA.; Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA., Heaton NS; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA.; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.; Duke Cancer Institute, Duke University School of Medicine, Durham, NC 27710, USA.

Publikováno v: Science signaling [Sci Signal] 2022 Oct 25; Vol. 15 (757), pp. eabm0808. Date of Electronic Publication: 2022 Oct 25.

The FDA-approved drug Alectinib compromises SARS-CoV-2 nucleocapsid phosphorylation and inhibits viral infection in vitro.

Autor: Yaron TM, Heaton BE, Levy TM, Johnson JL, Jordan TX, Cohen BM, Kerelsky A, Lin TY, Liberatore KM, Bulaon DK, Kastenhuber ER, Mercadante MN, Shobana-Ganesh K, He L, Schwartz RE, Chen S, Weinstein H, Elemento O, Piskounova E, Nilsson-Payant BE, Lee G, Trimarco JD, Burke KN, Hamele CE, Chaparian RR, Harding AT, Tata A, Zhu X, Tata PR, Smith CM, Possemato AP, Tkachev SL, Hornbeck PV, Beausoleil SA, Anand SK, Aguet F, Getz G, Davidson AD, Heesom K, Kavanagh-Williamson M, Matthews D, tenOever BR, Cantley LC, Blenis J, Heaton NS

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 Dec 16. Date of Electronic Publication: 2020 Dec 16.