Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Liangli Wang"'
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 11, Iss C, Pp 263-271 (2018)
The nuclear envelope is a physiological barrier to electrogene transfer. To understand different mechanisms of the nuclear entry for electrotransfected plasmid DNA (pDNA), the current study investigated how manipulation of the mechanisms could affect
Externí odkaz:
https://doaj.org/article/733d56f0bfa740a6943fad7ef44a885a
Publikováno v:
Cancers, Vol 12, Iss 9, p 2603 (2020)
T cell receptor (TCR) knockout is a critical step in producing universal chimeric antigen receptor T cells for cancer immunotherapy. A promising approach to achieving the knockout is to deliver the CRISPR/Cas9 system into cells using electrotransfer
Externí odkaz:
https://doaj.org/article/59e6184505c849c689bf50fd4c02015b
Publikováno v:
PLoS ONE, Vol 12, Iss 2, p e0171699 (2017)
A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene deliver
Externí odkaz:
https://doaj.org/article/c89c4e4c2f06430aa92c36fbf3af13aa
Publikováno v:
Diagnostic Cytopathology. 50:442-450
Core needle biopsy (CNB) and fine needle aspiration (FNA) are currently the most common biopsy methods for investigation of soft tissue lesions. Selection of the method to be used depends on a number of factors including diagnostic accuracy, local ex
Publikováno v:
Bioelectricity
BACKGROUND: Pulsed electric field has been widely used to facilitate molecular cargo transfer into cells. However, the cell viability is often decreased when trying to increase the electrotransfer efficiency. We hypothesize that the decrease is due t
Publikováno v:
Microscopy and Microanalysis. 24:553-563
Emerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastru
Publikováno v:
Cancers
Volume 12
Issue 9
Cancers, Vol 12, Iss 2603, p 2603 (2020)
Volume 12
Issue 9
Cancers, Vol 12, Iss 2603, p 2603 (2020)
T cell receptor (TCR) knockout is a critical step in producing universal chimeric antigen receptor T cells for cancer immunotherapy. A promising approach to achieving the knockout is to deliver the CRISPR/Cas9 system into cells using electrotransfer
Autor:
Fan Yuan, Liangli Wang, Jing Ji, Lisa D. Cervia, Adrian Pickar-Oliver, Charles A. Gersbach, Chun-Chi Chang, Mao Mao, Paloma B. Liton
Publikováno v:
Adv Biosyst
Cell engineering relies heavily on viral vectors for the delivery of molecular cargo into cells due to their superior efficiency compared to nonviral ones. However, viruses are immunogenic and expensive to manufacture, and have limited delivery capac
Publikováno v:
Molecular Therapy. Nucleic Acids
Molecular Therapy: Nucleic Acids, Vol 11, Iss C, Pp 263-271 (2018)
Molecular Therapy: Nucleic Acids, Vol 11, Iss C, Pp 263-271 (2018)
The nuclear envelope is a physiological barrier to electrogene transfer. To understand different mechanisms of the nuclear entry for electrotransfected plasmid DNA (pDNA), the current study investigated how manipulation of the mechanisms could affect
Autor:
Jianyong Huang, Katheryn E. Rothenberg, Chun-Chi Chang, Yingxiao Wang, Liangli Wang, Brenton D. Hoffman, Mao Mao, Fan Yuan, Paloma B. Liton
Publikováno v:
Molecular Therapy. 25:803-815
Electrotransfection is a widely used method for delivering genes into cells with electric pulses. Although different hypotheses have been proposed, the mechanism of electrotransfection remains controversial. Previous studies have indicated that uptak