Selective and predicable amine conjugation sites by kinetic characterization under excess reagents

Autor: Wei-Chun Huang, Li-Juan Huang, Liang-Sheng Hsu, Shih-Ting Huang, Wen-Ting Lo, Tzu-Fan Wang, Wei-Ting Sun, Win-Yin Wei, Ying-Shuan Lee, Shih-Hsien Chuang, Chao-Pin Lee, Ho-Hsuan Chou, Shu-Hui Chen

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)

Abstract The site selectivity for lysine conjugation on a native protein is difficult to control and characterize. Here, we applied mass spectrometry to examine the conjugation kinetics of Trastuzumab-IgG (Her-IgG) and α-lactalbumin under excess lin

Externí odkaz: https://doaj.org/article/ae57a14857544249a44e7cc16ab89855

Selective and predicable amine conjugation sites by kinetic characterization under excess reagents.

Autor: Huang, Wei-Chun¹ (AUTHOR), Huang, Li-Juan¹ (AUTHOR), Hsu, Liang-Sheng¹ (AUTHOR), Huang, Shih-Ting¹ (AUTHOR), Lo, Wen-Ting¹ (AUTHOR), Wang, Tzu-Fan² (AUTHOR), Sun, Wei-Ting³ (AUTHOR), Wei, Win-Yin³ (AUTHOR), Lee, Ying-Shuan³ (AUTHOR), Chuang, Shih-Hsien³ (AUTHOR), Lee, Chao-Pin^3,4 (AUTHOR), Chou, Ho-Hsuan¹ (AUTHOR) hohsuan@ncku.edu.tw, Chen, Shu-Hui¹ (AUTHOR) shchen@mail.ncku.edu.tw

Publikováno v: Scientific Reports. 10/27/2021, Vol. 11 Issue 1, p1-10. 10p.