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pro vyhledávání: '"Lew, Matthew D."'
We prove that it is impossible to distinguish two spatially overlapping fluorescent molecules from a single rotating molecule, even if one modulates the polarization of pumping light or the detection dipole-spread function (DSF). If the target is kno
Externí odkaz:
http://arxiv.org/abs/2406.04469
Autor:
Wu, Tingting, Lew, Matthew D.
Publikováno v:
Liang, J. (ed) Coded Optical Imaging. Springer, Cham. (2024)
Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to visualize their 3D
Externí odkaz:
http://arxiv.org/abs/2310.05810
Autor:
Jusuf, James M., Lew, Matthew D.
Publikováno v:
Opt. Express Vol. 30, Issue 20, pp. 37154-37174 (2022)
The past decade has brought many innovations in optical design for 3D super-resolution imaging of point-like emitters, but these methods often focus on single-emitter localization precision as a performance metric. Here, we propose a simple heuristic
Externí odkaz:
http://arxiv.org/abs/2111.14334
Autor:
King, Matthew R., Ruff, Kiersten M., Lin, Andrew Z., Pant, Avnika, Farag, Mina, Lalmansingh, Jared M., Wu, Tingting, Fossat, Martin J., Ouyang, Wei, Lew, Matthew D., Lundberg, Emma, Vahey, Michael D., Pappu, Rohit V.
Publikováno v:
In Cell 11 April 2024 187(8):1889-1906
Autor:
Zhang, Oumeng, Lew, Matthew D.
Publikováno v:
J. Opt. Soc. Am. A 38(2), 288-297 (2021)
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background ratio in typi
Externí odkaz:
http://arxiv.org/abs/2010.04064
Autor:
Zhang, Oumeng, Lew, Matthew D.
Publikováno v:
J. Opt. Soc. Am. A 38(2), 277-287 (2021)
Precisely measuring the three-dimensional position and orientation of individual fluorophores is challenging due to the substantial photon shot noise in single-molecule experiments. Facing this limited photon budget, numerous techniques have been dev
Externí odkaz:
http://arxiv.org/abs/2010.04060
Autor:
Zhang, Oumeng, Lew, Matthew D.
Publikováno v:
Phys. Rev. Research 2, 033114 (2020)
Precisely measuring molecular orientation is key to understanding how molecules organize and interact in soft matter, but the maximum theoretical limit of measurement precision has yet to be quantified. We use quantum estimation theory and Fisher inf
Externí odkaz:
http://arxiv.org/abs/2003.07846
Publikováno v:
2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019), 325 (2019)
In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully benefit from th
Externí odkaz:
http://arxiv.org/abs/1901.03898
Autor:
Zhang, Oumeng, Lew, Matthew D.
Publikováno v:
Phys. Rev. Lett. 122, 198301 (2019)
Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to appear to be m
Externí odkaz:
http://arxiv.org/abs/1811.09017
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