Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Lev Kotler"'
Publikováno v:
ELECTROPHORESIS. 23:3062-3070
The goal of this work was to reduce the capillary electrophoresis (CE) separation time of DNA sequencing fragments with linear polyacrylamide solutions while maintaining the previously achieved long read lengths of 1000 bases. Separation speed can be
Autor:
Arthur W. Miller, Lev Kotler, Haihong Zhou, Brett A. Buchholz, Zoran Sosic, Annelise E. Barron, Barry L. Karger
Publikováno v:
Analytical Chemistry. 72:1045-1052
This paper presents results on ultralong read DNA sequencing with relatively short separation times using capillary electrophoresis with replaceable polymer matrixes. In previous work, the effectiveness of mixed replaceable solutions of linear polyac
Autor:
Arthur W. Miller, Oscar Salas-Solano, Wolfgang Goetzinger, Barry L. Karger, Zoran Sosic, Emanuel Carrilho, Lev Kotler
Publikováno v:
Analytical Chemistry. 70:3996-4003
Long, accurate reads are an important factor for high-throughput de novo DNA sequencing. In previous work from this laboratory, a separation matrix of high-weight-average molecular mass (HMM) linear polyacrylamide (LPA) at a concentration of 2% (w/w)
Publikováno v:
Analytical Chemistry. 70:1516-1527
A method for the cleanup of Sanger DNA sequencing reaction products for capillary electrophoresis analysis with replaceable polymer solutions has been developed. A poly(ether sulfone) ultrafiltration membrane pretreated with linear polyacrylamide was
Autor:
Stephen F. McLaughlin, Martin G. Reese, Gina Costa, Francisco M. De La Vega, Cynthia L. Hendrickson, Can Alkan, Joel A. Malek, Tanya Sokolsky, Andrew MacBride, Alena A. Antipova, Kathleen C. Hayashibara, Zheng Zhang, Jonathan M. Manning, Alan Blanchard, Swati Ranade, Haoning Fu, Kevin McKernan, Adam Sannicandro, Damon S. Perez, Vineet Bafna, Jeremy R. Stuart, Michael P. Moore, Robert E. Beaudoin, Rajesh Gottimukkala, Yutao Fu, Lei Zhang, Eric F. Tsung, Andrew Sheridan, Cisyla Duncan, Ali Bashir, Fiona Hyland, Jeffrey M. Kidd, Jeffrey K. Ichikawa, Michael W. Laptewicz, Michael D. Rhodes, Brittany E. Coleman, Michael R. Lyons, Eileen T. Dimalanta, Clarence Lee, Shan Yang, Bin Li, Evan E. Eichler, Heather E. Peckham, Christopher Clouser, Lev Kotler
Publikováno v:
Genome research. 19(9)
We describe the genome sequencing of an anonymous individual of African origin using a novel ligation-based sequencing assay that enables a unique form of error correction that improves the raw accuracy of the aligned reads to >99.9%, allowing us to
Publikováno v:
Electrophoresis. 23(17)
The goal of this work was to reduce the capillary electrophoresis (CE) separation time of DNA sequencing fragments with linear polyacrylamide solutions while maintaining the previously achieved long read lengths of 1000 bases. Separation speed can be
Autor:
Hui, He, Brett A, Buchholz, Lev, Kotler, Arthur W, Miller, Annelise E, Barron, Barry L, Karger
Publikováno v:
Electrophoresis. 23(10)
Read length in DNA sequencing by capillary electrophoresis at elevated temperatures is shown to be greatly affected by the extent of hydrophobicity of the polymer separation matrix. At column temperatures of up to 80 degrees C, hydrophilic linear pol
Publikováno v:
Analytical chemistry. 70(8)
In the previous paper, a sample cleanup procedure for DNA sequencing reaction products was developed, in which template DNA was removed by ultrafiltration and the total concentration of salts (chloride and di- and deoxynucleotides) was decreased belo
Autor:
Emanuel Carrilho, Oscar Salas-Solano, Wolfgang Goetzinger, Lev Kotler, Barry L. Karger, Marie C. Ruiz-Martinez
Publikováno v:
Electrophoresis. 19(2)
In a previous paper, a 2% w/w replaceable high molecular mass linear polyacrylamide solution (high molecular mass LPA) was used to achieve long read-lengths for DNA sequencing by capillary electrophoresis (E. Carrilho et al., Anal. Chem. 1996, 68, 33
Autor:
Lev Kotler, Jeffrey Kesilman, Marie C. Ruiz-Martinez, Emanuel Carrilho, Barry L. Karger, Arthur W. Miller
Publikováno v:
SPIE Proceedings.
Factors to be considered for robust high-throughput automated dna sequencing using a multiple-capillary array instrumentEmanuel Carri1ho, Arthur W. Miller, Marie C. RuizMartinezt, Lev Kotler, Jeffrey Kesilman, and Barry L. Karger*Northeastern Univers