Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Lev A. Sukovatyi"'
Autor:
Darya P. Surzhikova, Lev A. Sukovatyi, Elena V. Nemtseva, Elena N. Esimbekova, Evgenia A. Slyusareva
Publikováno v:
Micromachines, Vol 14, Iss 7, p 1442 (2023)
In this work, we considered the influence of viscogenic agents (glycerol, sucrose) as well as the temperature on the fluorescent characteristics of fluorescein at pH 6.5 in order to describe the acid-base status of local environment in terms of a spe
Externí odkaz:
https://doaj.org/article/6656d6b97a4c4592998046e5c5ce03ca
Autor:
Albert E. Lisitsa, Lev A. Sukovatyi, Anna A. Deeva, Dmitry V. Gulnov, Elena N. Esimbekova, Valentina A. Kratasyuk, Elena V. Nemtseva
Publikováno v:
Life, Vol 13, Iss 6, p 1384 (2023)
A complex heterogeneous intracellular environment seems to affect enzymatic catalysis by changing the mobility of biomolecules, their stability, and their conformational states, as well as by facilitating or hindering continuously occurring interacti
Externí odkaz:
https://doaj.org/article/a706534799e74a5695c6ff2a387152cb
Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect
Autor:
Anna A. Deeva, Albert E. Lisitsa, Lev A. Sukovatyi, Tatiana N. Melnik, Valentina A. Kratasyuk, Elena V. Nemtseva
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 15, p 8119 (2022)
The evaluation of temperature effects on the structure and function of enzymes is necessary to understand the mechanisms underlying their adaptation to a constantly changing environment. In the current study, we investigated the influence of temperat
Externí odkaz:
https://doaj.org/article/68f0a87e09a44df9865889089da56a84
Autor:
Elena V. Nemtseva, Dmitry V. Gulnov, Marina A. Gerasimova, Lev A. Sukovatyi, Ludmila P. Burakova, Natalya E. Karuzina, Bogdan S. Melnik, Valentina A. Kratasyuk
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 19, p 10449 (2021)
Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite
Externí odkaz:
https://doaj.org/article/e5f78c30609d40cc926ad79fec6260a4
Autor:
Albert E. Lisitsa, Lev A. Sukovatyi, Sergey I. Bartsev, Anna A. Deeva, Valentina A. Kratasyuk, Elena V. Nemtseva
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 16, p 8827 (2021)
Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulato
Externí odkaz:
https://doaj.org/article/9fe2a150e6644c5896c64fff8457ebd5
Autor:
Nemtseva, Albert E. Lisitsa, Lev A. Sukovatyi, Anna A. Deeva, Dmitry V. Gulnov, Elena N. Esimbekova, Valentina A. Kratasyuk, Elena V.
Publikováno v:
Life; Volume 13; Issue 6; Pages: 1384
A complex heterogeneous intracellular environment seems to affect enzymatic catalysis by changing the mobility of biomolecules, their stability, and their conformational states, as well as by facilitating or hindering continuously occurring interacti
Publikováno v:
Biophysics. 65:966-971
The effects of viscous media with glycerol and sucrose (10–40%) on the kinetics of the bacterial bioluminescent reaction have been investigated by stopped-flow technique. Increment of quantum yield in media with 10% of both osmolytes was shown. Hig
Autor:
Dmitry V. Gulnov, Elena V. Nemtseva, Ludmila Burakova, Bogdan S. Melnik, Natalya E. Karuzina, Lev A. Sukovatyi, Valentina A. Kratasyuk, M. A. Gerasimova
Publikováno v:
International Journal of Molecular Sciences
Volume 22
Issue 19
International Journal of Molecular Sciences, Vol 22, Iss 10449, p 10449 (2021)
Volume 22
Issue 19
International Journal of Molecular Sciences, Vol 22, Iss 10449, p 10449 (2021)
Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite
Autor:
Sergey I. Bartsev, Albert E. Lisitsa, Lev A. Sukovatyi, Valentina A. Kratasyuk, Elena V. Nemtseva, Anna A. Deeva
Publikováno v:
International Journal of Molecular Sciences
Volume 22
Issue 16
International Journal of Molecular Sciences, Vol 22, Iss 8827, p 8827 (2021)
Volume 22
Issue 16
International Journal of Molecular Sciences, Vol 22, Iss 8827, p 8827 (2021)
Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulato
Publikováno v:
Doklady. Biochemistry and biophysics. 492(1)
The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1-1.3 cP) the decay rate rises, wh