Výsledky vyhledávání

CRaTER enrichment for on-target gene-editing enables generation of variant libraries in hiPSCs

Autor: Clayton E. Friedman, Shawn Fayer, Sriram Pendyala, Wei-Ming Chien, Linda Tran, Leslie Chao, Ashley Mckinstry, Elaheh Karbassi, Aidan M. Fenix, Alexander Loiben, Charles E. Murry, Lea M. Starita, Douglas M. Fowler, Kai-Chun Yang

Publikováno v: bioRxiv

SUMMARYStandard transgenic cell line generation requires screening 100-1000s of colonies to isolate correctly edited cells. We describeCRISPRaOn-TargetEditingRetrieval (CRaTER) which enriches for cells with on-target knock-in of a cDNA-fluorescent re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44853a4cf343a890681fde377732f725
https://doi.org/10.1101/2023.01.25.525582

Zobrazit plný text záznamu

Abstract P2110: Myosin-7 E848G Mutation Reduces Contractility And Activates P53-associated Intrinsic Apoptosis In Patient-derived Induced Pluripotent Stem Cell Model Of Hypertrophic Cardiomyopathy

Autor: Alexander Loiben, Clayton Friedman, Wei-Ming Chen, Leslie Chao, Kai Chun Yang

Publikováno v: Circulation Research. 131

Introduction: Familial hypertrophic cardiomyopathy (HCM), affecting 1 in 500 adults, is characterized by idiopathic thickening of the heart and impaired systolic function. Mechanisms through which cardiac sarcomeric mutations manifest in HCM are poor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d5ca51eac086a195d6ca1a85ef9d09fa
https://doi.org/10.1161/res.131.suppl_1.p2110

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání