Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Leslie C. L. Chan"'
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e36324 (2012)
The Heliothine insect complex contains some of the most destructive pests of agricultural crops worldwide, including the closely related Helicoverpa zea and H. armigera. Biological control using baculoviruses is practiced at a moderate level worldwid
Externí odkaz:
https://doaj.org/article/6b0681b242af4f28a76dceb558693062
Publikováno v:
Applied Microbiology and Biotechnology. 100:9239-9253
Baculoviruses are recognized as viral workhorses of biotechnology, being used for production of vaccines, complex recombinant proteins, gene delivery vectors' and safe biological pesticides. Improving production yields and understanding the interacti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b68fae7731c1bae22e1b69039a973c5
https://doi.org/10.1007/s00253-016-7774-3
https://doi.org/10.1007/s00253-016-7774-3
Publikováno v:
Archives of Virology. 159:91-102
The performance of bioprocesses involving baculoviruses largely depends on an efficient infection of cells by concentrated budded virus (BV) inoculums. Baculovirus expression vector systems have been established using Autographa californica nucleopol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26e06cdefca7e449642daca992b13772
https://doi.org/10.1007/s00705-013-1789-1
https://doi.org/10.1007/s00705-013-1789-1
Publikováno v:
Applied Microbiology and Biotechnology. 97:5245-5257
The cell density effect is a well-established constraint in the baculovirus-insect cell expression platform, in which cell-specific productivity declines with increasing cell density, hence limiting the maximum achievable volumetric yield of protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa6cd62bbfc1fe2299f2db4038a13599
https://doi.org/10.1007/s00253-013-4835-8
https://doi.org/10.1007/s00253-013-4835-8
Autor:
Steven, Reid, Leslie C L, Chan, Leila, Matindoost, Charlotte, Pushparajan, Gabriel, Visnovsky
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1477
While large-scale culture of insect cells will need to be conducted using bioreactors up to 10,000 l scale, many of the main challenges for cell culture-based production of insecticidal viruses can be studied using small-scale (20-500 ml) shaker/spin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::31a97e24a9efd8cce1d5ab6be9591faa
https://pubmed.ncbi.nlm.nih.gov/27565495
https://pubmed.ncbi.nlm.nih.gov/27565495
Autor:
Steven Reid, Trinh T. B. Tran, Hoai T. Huynh, Leslie C. L. Chan, Lars K. Nielsen, Stefanie Dietmair
Publikováno v:
Methods. 56:396-407
Metabolomics refer to the global analysis of small molecule metabolites in a biological system, and can be a powerful tool to elucidate and optimize cellular processes, particularly when integrated into a systems biology framework. Determining the en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fa9f36600b16ad58ca9bd7363373f1c
https://doi.org/10.1016/j.ymeth.2011.11.009
https://doi.org/10.1016/j.ymeth.2011.11.009
Autor:
Aaron H. Colby, Steven Reid, G. Seth Roberts, Sam Yu, Mark W. Grinstaff, Qinglu Zeng, Will Anderson, Robert Vogel, Leslie C. L. Chan
Publikováno v:
Biosensors and Bioelectronics. 31:17-25
Scanning ion occlusion sensing (SIOS), a technique that uses a tunable pore to detect the passage of individual nano-scale objects, is applied here for the rapid, accurate and direct measurement of synthetic and biological nanoparticle concentrations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::061e808c61ff7f83b6367c0f17d10c08
https://doi.org/10.1016/j.bios.2011.09.040
https://doi.org/10.1016/j.bios.2011.09.040
Publikováno v:
Journal of Virological Methods. 175:197-205
Baculovirus pesticides are increasingly being used as effective biological control agents against caterpillar pests worldwide. Increasing occlusion body (OB) yields per cell in culture is the main challenge to enable commercialization of in vitro pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ff399cf8a441c422879483bd2cf22bd
https://doi.org/10.1016/j.jviromet.2011.05.011
https://doi.org/10.1016/j.jviromet.2011.05.011
Publikováno v:
Biotechnology Progress. 27:614-624
Large-scale commercialization of baculovirus biopesticides for the control of insect pests requires a cell culture production process, and knowledge of the infection kinetics is a vital prerequisite for process optimization. Well-characterized kineti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5f5eb15dfacd6e2dfb79fe29d5774b4
https://doi.org/10.1002/btpr.602
https://doi.org/10.1002/btpr.602
Autor:
Leslie C L, Chan, Steven, Reid
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1350
Baculovirus-based Insect Cell Technology (ICT) is widely used for the expression of recombinant heterologous proteins and baculovirus bioinsecticides, and has recently gained momentum as a commercial manufacturing platform for human and veterinary va
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::93edf24ff6b9ee2e4fbfac2b69349fb6
https://pubmed.ncbi.nlm.nih.gov/26820858
https://pubmed.ncbi.nlm.nih.gov/26820858