Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Lesley C. Jones"'
Autor:
William Chour, Jongchan Choi, Jingyi Xie, Mary E. Chaffee, Thomas M. Schmitt, Kathryn Finton, Diana C. DeLucia, Alexander M. Xu, Yapeng Su, Daniel G. Chen, Rongyu Zhang, Dan Yuan, Sunga Hong, Alphonsus H. C. Ng, Jonah Z. Butler, Rick A. Edmark, Lesley C. Jones, Kim M. Murray, Songming Peng, Guideng Li, Roland K. Strong, John K. Lee, Jason D. Goldman, Philip D. Greenberg, James R. Heath
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-13 (2023)
Abstract The discovery and characterization of antigen-specific CD8+ T cell clonotypes typically involves the labor-intensive synthesis and construction of peptide-MHC tetramers. We adapt single-chain trimer (SCT) technologies into a high throughput
Externí odkaz:
https://doaj.org/article/3bdea162be0a4447904d5af92d6a6730
Autor:
David Rabuka, Robyn M. Barfield, Jeanne Baker, Gregory W. de Hart, Stefanie Bañas, Penelope M. Drake, Patrick G. Holder, Lesley C. Jones
Publikováno v:
The Journal of Biological Chemistry
Background: Aerobic formylglycine-generating enzyme (FGE) converts cysteine to formylglycine in vivo. Results: Purified FGE requires preactivation with copper to convert cysteine to formylglycine in vitro. Conclusion: FGE is a metalloenzyme. It is al
Autor:
Robyn M. Barfield, Gregory W. de Hart, Jesse M. McFarland, David Rabuka, Albert W. Garofalo, Penelope M. Drake, Stefanie Bañas, Patrick G. Holder, Lesley C. Jones, Romas Alvydas Kudirka, Aaron Edward Albers
Publikováno v:
Chemistry & Biology. 22:293-298
SummaryThere is a need for facile chemistries that allow for chemo- and regioselectivity in bioconjugation reactions. To address this need, we are pioneering site-specific bioconjugation methods that use formylglycine as a bioorthogonal handle on a p
Autor:
Jeanne Baker, David Rabuka, Wes Zmolek, Jesse M. McFarland, Penelope M. Drake, Robyn M. Barfield, Lesley C. Jones, Abhijit Bhat, Albert W. Garofalo, Gregory W. de Hart, Stefanie Bañas, Romas Alvydas Kudirka, Aaron Edward Albers, Patrick G. Holder
Publikováno v:
Bioconjugate Chemistry
It is becoming increasingly clear that site-specific conjugation offers significant advantages over conventional conjugation chemistries used to make antibody–drug conjugates (ADCs). Site-specific payload placement allows for control over both the
Autor:
Gregory T. Bleck, Patrick G. Holder, David Rabuka, Lesley C. Jones, Penelope M. Drake, Dona York, Jeanne Baker, Robyn M. Barfield
Publikováno v:
BMC Biotechnology
Background The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic research and drug development. F
Autor:
David Rabuka, Robyn M. Barfield, Gregory W. de Hart, Penelope M. Drake, Lesley C. Jones, Aaron Edward Albers, Paresh Agarwal, Romas Alvydas Kudirka
Publikováno v:
Bioconjugate chemistry. 24(6)
Aldehyde- and ketone-functionalized biomolecules have found widespread use in biochemical and biotechnological fields. They are typically conjugated with hydrazide or aminooxy nucleophiles under acidic conditions to yield hydrazone or oxime products