High-throughput screen using fluorescence lifetime detects compounds that modulate myosin-binding protein C interactions with actin

Autor: Thomas A. Bunch, Piyali Guhathakurta, Lepak Vc, Kanassatega R, Andrew R. Thompson, Brett A. Colson, Anna Wilson, David D. Thomas

Cardiac myosin-binding protein C (cMyBP-C) interacts with actin and myosin to modulate cardiac contractility. These interactions are regulated by cMyBP-C phosphorylation. Heart failure patients often have decreased cMyBP-C phosphorylation and phospho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::89aefe73f4c490bc2e4c15bc6eb952c3
https://doi.org/10.1101/2021.03.24.436879

Drug discovery for heart failure targeting myosin-binding protein C.

Autor: Bunch TA; Department of Cellular & Molecular Medicine, University of Arizona, Tucson, Arizona, USA., Guhathakurta P; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA., Thompson AR; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA., Lepak VC; Department of Cellular & Molecular Medicine, University of Arizona, Tucson, Arizona, USA., Carter AL; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA., Thomas JJ; Photonic Pharma LLC, Minneapolis, Minnesota, USA., Thomas DD; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA; Photonic Pharma LLC, Minneapolis, Minnesota, USA. Electronic address: ddt@umn.edu., Colson BA; Department of Cellular & Molecular Medicine, University of Arizona, Tucson, Arizona, USA. Electronic address: bcolson@arizona.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2023 Dec; Vol. 299 (12), pp. 105369. Date of Electronic Publication: 2023 Oct 20.

Human cardiac myosin-binding protein C phosphorylation- and mutation-dependent structural dynamics monitored by time-resolved FRET.

Autor: Kanassatega RS; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, United States of America., Bunch TA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, United States of America., Lepak VC; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, United States of America., Wang C; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, United States of America., Colson BA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, United States of America. Electronic address: bcolson@arizona.edu.

Publikováno v: Journal of molecular and cellular cardiology [J Mol Cell Cardiol] 2022 May; Vol. 166, pp. 116-126. Date of Electronic Publication: 2022 Feb 25.

Age-specific treatment effects of orexin/hypocretin-receptor antagonism on methamphetamine-seeking behavior.

Autor: Zlebnik NE; Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA. Electronic address: nzlebnik@som.umaryland.edu., Holtz NA; Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA., Lepak VC; Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA., Saykao AT; Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA., Zhang Y; Research Triangle Institute, Research Triangle Park, NC, 27709, USA., Carroll ME; Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA.

Publikováno v: Drug and alcohol dependence [Drug Alcohol Depend] 2021 Jul 01; Vol. 224, pp. 108719. Date of Electronic Publication: 2021 Apr 27.

A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin.

Autor: Bunch TA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ., Lepak VC; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ., Bortz KM; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ., Colson BA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ.

Publikováno v: The Journal of general physiology [J Gen Physiol] 2021 Mar 01; Vol. 153 (3).