Zobrazeno 1 - 10 of 15 pro vyhledávání: '"Leonidas G. Theodorou"'
1
Akademický článek
Aspects on the catalysis of lipase from porcine pancreas (type VI-s) in aqueous media: development of ion-pairs
Autor: Marina Kokkinou, Leonidas G. Theodorou, Emmanuel M. Papamichael
Publikováno v: Brazilian Archives of Biology and Technology, Vol 55, Iss 2, Pp 231-236 (2012)
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from porcine pancreas, type VI-s (PPL), in hydrolyzing an ester substrate in aqueous media. The conclusions were based on the pH-profiles of Michaelis-Ment
Externí odkaz: https://doaj.org/article/aa3defe0e56c4c6186a042d544ab1bce
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2
Akademický článek
Necessary auxiliary background for efficient use of an existing computer program of non-parametric fitting of nonlinear equations
Autor: Emmanuel M. Papamichael, Leonidas G. Theodorou
Publikováno v: Brazilian Archives of Biology and Technology, Vol 52, Iss 2, Pp 437-448 (2009)
This work constituted a significant contribution for more efficient use of a valuable computer program of non-parametric fitting of nonlinear multiparametric equations to experimental data. However, prerequisite in this context was the transformation
Externí odkaz: https://doaj.org/article/5f82fc2139654d7aba5976b1aaf3c283
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3
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
Autor: Emmanuel M. Papamichael, Panagiota-Yiolanda Stergiou, Athanasios Foukis, Maria Papagianni, Leonidas G. Theodorou
Publikováno v: Brazilian Archives of Biology and Technology, Vol 57, Iss 3, Pp 421-426 (2014)
Brazilian Archives of Biology and Technology v.57 n.3 2014
Brazilian Archives of Biology and Technology
Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
Brazilian Archives of Biology and Technology, Volume: 57, Issue: 3, Pages: 421-426, Published: JUN 2014
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventio
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7e072952a0a28f5f16fb8f11ec2feb5
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016&lng=en&tlng=en
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4
Advances in lipase-catalyzed esterification reactions
Autor: Athanasios Foukis, Maria Parapouli, Ashok Pandey, Michalis Filippou, Maria Koukouritaki, Amalia S. Afendra, Panagiota-Yiolanda Stergiou, Leonidas G. Theodorou, Efstathios Hatziloukas, Emmanuel M. Papamichael
Publikováno v: Biotechnology Advances. 31:1846-1859
Lipase-catalyzed esterification reactions are among the most significant chemical and biochemical processes of industrial relevance. Lipases catalyze hydrolysis as well as esterification reactions. Enzyme-catalyzed esterification has acquired increas
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23ae9ab1a35fbb74d29f4f39488b2ffd
https://doi.org/10.1016/j.biotechadv.2013.08.006
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6
Purification and characterization of a novel extracellular protease from a halo‐alkaliphilicBacillussp.17N‐1,active in polar organic solvents
Autor: Emmanuel M. Papamichael, Leonidas G. Theodorou, Angelos Perisynakis, Constantin Drainas
Publikováno v: Environmental Technology. 31:1073-1082
A novel enzyme of molecular mass about 29 kDa was purified from the strain halo-alkaliphilic Bacillus sp. 17N-1 and designated protease-B-17N-1. This enzyme is likely to be a cysteine protease; it was found active in media containing EDTAK2 and dithi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a2e695958b4867932b606a9a31f93b1
https://doi.org/10.1080/09593331003664136
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7
Proton Inventories Constitute Reliable Tools in Investigating Enzymatic Mechanisms: Application on a Novel Thermo-stable Extracellular Protease from a Halo-Alkalophilic Bacillus sp
Autor: Emmanuel M. Papamichael, Constantin Drainas, Angelos Perisynakis, Leonidas G. Theodorou, Krystalenia Valasaki
Publikováno v: Journal of Biochemistry. 142:293-300
A novel protease designated protease-A-17N-1, was purified from the haloalkalophilic Bacillus sp. 17N-1, and found active in media containing dithiothreitol and EDTAK(2). This enzyme maintained significant activity from pH 6.00 to 9.00, showed optimu
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a2b9938ebc15596f1cfa1f62de05dd2
https://doi.org/10.1093/jb/mvm132
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8
Insight Into Catalytic Mechanism of Papain-Like Cysteine Proteinases: The Case of D158
Autor: Leonidas G. Theodorou, J. G. Bieth, Emmanuel M. Papamichael
Publikováno v: Applied Biochemistry and Biotechnology. 118:171-176
We studied the role of D158 in papain-like cysteine proteinases by using subtilisin Carlsberg, and its chemically modified analog thiolsubtilisin, by applying the proton inventory (PI) method and also by taking into account the pH profiles of the k c
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::631076c7046bb17ede6e5dd99a2e4e42
https://doi.org/10.1385/abab:118:1-3:171
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9
Effective Kinetic Methods and Tools in Investigating the Mechanism of Action of Specific Hydrolases
Autor: Athanasios Foukis, Emmanuel M. Papamichael, Marina Kokkinou, Panagiota-Yiolanda Stergiou, Leonidas G. Theodorou
Publikováno v: Medicinal Chemistry and Drug Design
The investigation and identification of a reaction mechanism is essential in the sense that it provides information as to the molecular species of the reaction, and it is strongly based on the number and types of the phases involved; however, this di
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0456896bd012d1f5c984a40e4cead391
https://doi.org/10.5772/37070
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10
Purification, kinetic characterization and properties of a novel thermo-tolerant extracellular protease from Kluyveromyces marxianus IFO 0288 with potential biotechnological interest
Autor: Panagiota-Yiolanda Stergiou, Athanasios Foukis, Leonidas G. Theodorou, Emmanuel M. Papamichael, Maria Papagianni
Publikováno v: Bioresource technology. 123
A novel extracellular hydrolase of ∼45 kDa molecular mass was purified from Kluyveromyces marxianus IFO 0288 cultures and characterized as serine protease. The Km-value of protease (designated protease-KM-IFO-0288-A), which was found active in medi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3eac62d8b7e3d4bfd72c6903089009e
https://pubmed.ncbi.nlm.nih.gov/22940322
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