Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Leonhard Jakob"'
Autor:
Sarah Willkomm, Leonhard Jakob, Kevin Kramm, Veronika Graus, Julia Neumeier, Gunter Meister, Dina Grohmann
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.
Externí odkaz:
https://doaj.org/article/fc70d6bcbb934f14a1ea17f995f8ffda
Publikováno v:
Biochemistry and Biophysics Reports, Vol 17, Iss , Pp 1-9 (2019)
Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules. Site-specific labelling of a protein is of particular interest for fluorescence-based
Externí odkaz:
https://doaj.org/article/d3d133e96974470191dc4970d533c30a
Autor:
Inga Loedige, Leonhard Jakob, Thomas Treiber, Debashish Ray, Mathias Stotz, Nora Treiber, Janosch Hennig, Kate B. Cook, Quaid Morris, Timothy R. Hughes, Julia C. Engelmann, Michael P. Krahn, Gunter Meister
Publikováno v:
Cell Reports, Vol 13, Iss 6, Pp 1206-1220 (2015)
TRIM-NHL proteins are conserved among metazoans and control cell fate decisions in various stem cell linages. The Drosophila TRIM-NHL protein Brain tumor (Brat) directs differentiation of neuronal stem cells by suppressing self-renewal factors. Brat
Externí odkaz:
https://doaj.org/article/d97668f2c0d1480d89e8bdf2001c06c2
Publikováno v:
Nucleic Acids Research
The widespread and versatile prokaryotic CRISPR–Cas systems (clustered regularly interspaced short palindromic repeats and associated Cas proteins) constitute powerful weapons against foreign nucleic acids. Recently, the single-effector nuclease Ca
Autor:
Andreas Schmidbauer, Dina Grohmann, Marius Rutkauskas, Fabian Welzel, Leonhard Jakob, Ralf Seidel, Fergus Fettes, Pierre Aldag
Publikováno v:
Nucleic Acids Research
CRISPR-Cas9 is a ribonucleoprotein complex that sequence-specifically binds and cleaves double-stranded DNA. Wildtype Cas9 as well as its nickase and cleavage-incompetent mutants have been used in various biological techniques due to their versatilit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7719abb22b169fccf05e5cedcd11ca72
https://doi.org/10.1101/2021.08.04.455019
https://doi.org/10.1101/2021.08.04.455019
Publikováno v:
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports, Vol 17, Iss, Pp 1-9 (2019)
Biochemistry and Biophysics Reports, Vol 17, Iss, Pp 1-9 (2019)
Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules. Site-specific labelling of a protein is of particular interest for fluorescence-based
Publikováno v:
Nucleic Acids Research
Autor:
Mathias Stotz, Ludwig Wankerl, Gunter Meister, Stefan Hannus, Alexander Gust, Kevin Kramm, Dina Grohmann, Thomas Treiber, Kerrin Hansen, Leonhard Jakob, Franz Herzog, Nora Treiber
Publikováno v:
RNA. 22:383-396
In the microRNA (miRNA) pathway, Dicer processes precursors to mature miRNAs. For efficient processing, double-stranded RNA-binding proteins support Dicer proteins. In flies, Loquacious (Loqs) interacts with Dicer1 (dmDcr1) to facilitate miRNA proces
Publikováno v:
Nanoscale. 10(35)
Fluorescence resonance energy transfer (FRET) has been instrumental in determining the structure and dynamics of biomolecules but distances above 8 nanometers are not accessible. However, with the advent and rapid development of super-resolution (SR)
Autor:
Alexander Gust, Philip Tinnefeld, Daniela M. Zeitler, Dina Grohmann, Leonhard Jakob, Kevin Kramm, Astrid Bruckmann, Gunter Meister, Sarah Willkomm
Publikováno v:
Chembiochem : a European journal of chemical biology. 19(8)
Human cells are complex entities in which molecular recognition and selection are critical for cellular processes often driven by structural changes and dynamic interactions. Biomolecules appear in different chemical states, and modifications, such a