Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Leonard Spero"'
Analysis of the near-ultraviolet circular dichroic spectra of staphylococcal enterotoxins A, B and C
Autor:
Leonard Spero
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 671:193-201
The near-ultraviolet CD spectra from 260 to 300 nm of staphylococcal enterotoxins A, B and C were resolved empirically into Gaussian curves. Each spectrum contained the same six components with maximum ellipticities at virtually identical wavelengths
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::315df81f1c950bd6773e0727b0d487c5
https://doi.org/10.1016/0005-2795(81)90134-3
https://doi.org/10.1016/0005-2795(81)90134-3
Autor:
Leonard Spero, Anna D. Johnson-Winegar
Publikováno v:
Current Microbiology. 8:311-315
Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel. The main components had an isoionic point of pH=7.0 while other components ranged from pI=4.5 to pI=8.5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67eecd932b5f78e4e3d881426bb5ad1c
https://doi.org/10.1007/bf01573700
https://doi.org/10.1007/bf01573700
Autor:
Beverly A. Morlock, Leonard Spero
Publikováno v:
Journal of Biological Chemistry. 253:8787-8791
Staphylococcal entertoxin C1 is converted to a doubly cleaved molecule by trypsin digestion with one of the scissions internal to the disulfide loop and one external to it. The larger, disulfide-containing polypeptide (Mr = 22,000) exhibited excellen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68c880e92f7d77324b2859570e99af5f
https://doi.org/10.1016/s0021-9258(17)34246-1
https://doi.org/10.1016/s0021-9258(17)34246-1
Autor:
Leonard Spero, Beverly A. Morlock
Publikováno v:
The Journal of Immunology. 122:1285-1289
The strong cross-reactions demonstrated for staphylococcal enterotoxins B (SEB) and C1 (SEC1) by measurement of antigen-binding capacity were reflected in well defined polypeptides obtained by limited tryptic digestion from SEB and SEC1. Two antigeni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c34353667e48aedbed962d780312491a
https://doi.org/10.4049/jimmunol.122.4.1285
https://doi.org/10.4049/jimmunol.122.4.1285
Purification and characterization of different types of exfoliative toxin from Staphylococcus aureus
Publikováno v:
Infection and Immunity. 24:679-684
Exfoliative toxin was isolated from strain DI of Staphylococcus aureus using carboxymethyl cellulose and hydroxylapatite chromatography. This purified toxin was compared with that produced by strain TA. The specific biological activity of the two tox
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dca37d79ead8f6091951f857e2d43bb0
https://doi.org/10.1128/iai.24.3.679-684.1979
https://doi.org/10.1128/iai.24.3.679-684.1979
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 336:79-85
Four components have been demonstrated in staphylococcal enterotoxin B by isoelectric focusing in polyacrylamide gels. In a typical preparation their relative concentrations from the most to least cathodic were 6, 56, 31 and 7%. The components were n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68987b6eef55a0545631d5dd9da12ade
https://doi.org/10.1016/0005-2795(74)90386-9
https://doi.org/10.1016/0005-2795(74)90386-9
Publikováno v:
The Journal of Immunology. 120:86-89
Strong cross-reactions were demonstrated for staphylococcal enterotoxins B (SEB) and C1 (SEC1) by antigenbinding capacity and by competitive binding ability. Both SEB and SEC1 combined completely with the heterologous antibody although requiring four
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91b20e36bf38cdc16e8ac3c91a5784fe
https://doi.org/10.4049/jimmunol.120.1.86
https://doi.org/10.4049/jimmunol.120.1.86
Publikováno v:
Infection and Immunity. 30:381-384
Exfoliative toxin from Staphylococcus aureus was mitogenic for murine spleen cells. It was primarily active on T lymphocytes but also significantly stimulated B cells from the spleens of nude mice. The mitogenicity was not affected by simple sugar or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12a7dbed97658bc35c165461da932a8d
https://doi.org/10.1128/iai.30.2.381-384.1980
https://doi.org/10.1128/iai.30.2.381-384.1980
Publikováno v:
Infection and Immunity. 12:93-97
Staphylococcus aureus enterotoxins C1 (SEC1) and C2 (SEC2) produced from 50-liter quantities of crude culture supernatants were purified chromatographically in a neutral or acid milieu. Microheterogenity of SEC1 was markedly increased by treatment of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7378e3253ea8cf23cb0442cbb8e9814c
https://doi.org/10.1128/iai.12.1.93-97.1975
https://doi.org/10.1128/iai.12.1.93-97.1975
Autor:
Leonard Spero
Publikováno v:
Archives of Biochemistry and Biophysics. 73:484-491
In contrast to early observations on crude solutions of toxin, the purified toxin from Cl. botulinum, type A, was found to be quite stable at moderate alkalinity. The rate of inactivation increased by a factor of 4 × 104 over one pH unit above pH 10
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a13a87bcfe58f0bcbf020201af2f6e2c
https://doi.org/10.1016/0003-9861(58)90291-1
https://doi.org/10.1016/0003-9861(58)90291-1