QUAS-R: An SLC1A5-mediated glutamine uptake assay with single-cell resolution reveals metabolic heterogeneity with immune populations

Autor: Leonard R. Pelgrom, Gavin M. Davis, Simon O’Shaughnessy, Emilie J.M. Wezenberg, Sander I. Van Kasteren, David K. Finlay, Linda V. Sinclair

Publikováno v: Cell Reports, Vol 42, Iss 8, Pp 112828- (2023)

Summary: System-level analysis of single-cell data is rapidly transforming the field of immunometabolism. Given the competitive demand for nutrients in immune microenvironments, there is a need to understand how and when immune cells access these nut

Externí odkaz: https://doaj.org/article/f8ed02eb6f6c4fc3b2176333693c7e6f

mTORC1 signaling in antigen-presenting cells of the skin restrains CD8+ T cell priming

Autor: Leonard R. Pelgrom, Thiago A. Patente, Frank Otto, Lonneke V. Nouwen, Arifa Ozir-Fazalalikhan, Alwin J. van der Ham, Hendrik J.P. van der Zande, Graham A. Heieis, Ramon Arens, Bart Everts

Publikováno v: Cell Reports, Vol 40, Iss 1, Pp 111032- (2022)

Summary: How mechanistic target of rapamycin complex 1 (mTORC1), a key regulator of cellular metabolism, affects dendritic cell (DC) metabolism and T cell-priming capacity has primarily been investigated in vitro, but how mTORC1 regulates this in viv

Externí odkaz: https://doaj.org/article/af5a88d5847d42b5978559763a00c5ea

Metabolic sensor AMPK licenses CD103+dendritic cells to induce Treg responses

Autor: Thiago A Patente, Eline C Brombacher, Graham A Heieis, Leonard R Pelgrom, Anna Zawistowska-Deniziak, Frank Otto, Arifa Ozir-Fazalalikhan, Alwin J van der Ham, Bruno A Guigas, José AM Barbuto, Bart Everts

Dendritic cells (DCs) play a crucial role in promoting tolerance through priming of regulatory T cells (Treg). Several studies indicate DC tolerogenicity is dependent on catabolic metabolism. However, the role of AMP-activated Kinase (AMPK), a key en

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::591ea410013e3f3000f4a1242478ce0f
https://doi.org/10.1101/2023.02.21.528293

Soluble mannose receptor induces proinflammatory macrophage activation and metaflammation

Autor: Bart Everts, Leonard R. Pelgrom, Joost M. Lambooij, Isabel Stoetzel, Maria Embgenbroich, Joachim L. Schultze, Vanessa van Harmelen, Jonas Schulte-Schrepping, Hendrik J. P. van der Zande, Hanno Pijl, Kristian Händler, Lisa R. Hoving, Laura Schlautmann, Karin de Ruiter, Sven Burgdorf, Anna Zawistowska-Deniziak, Leonie Hussaarts, Ko Willems van Dijk, Bruno Guigas, Maria Yazdanbakhsh, Marjolein A. Wijngaarden, Noemí García-Tardón

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America 118(31), e2103304118 (2021). doi:10.1073/pnas.2103304118
Proc Natl Acad Sci U S A
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences, 118(31). NATL ACAD SCIENCES

Proinflammatory activation of macrophages in metabolic tissues is critically important in the induction of obesity-induced metaflammation. Here, we demonstrate that the soluble mannose receptor (sMR) plays a direct functional role in both macrophage

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd4853dd491738c9d4b4c81a0e54327c
https://hdl.handle.net/1887/3214620

mTORC1 signaling in antigen-presenting cells of the skin restrains CD8

Autor: Leonard R, Pelgrom, Thiago A, Patente, Frank, Otto, Lonneke V, Nouwen, Arifa, Ozir-Fazalalikhan, Alwin J, van der Ham, Hendrik J P, van der Zande, Graham A, Heieis, Ramon, Arens, Bart, Everts

Publikováno v: Cell reports. 40(1)

How mechanistic target of rapamycin complex 1 (mTORC1), a key regulator of cellular metabolism, affects dendritic cell (DC) metabolism and T cell-priming capacity has primarily been investigated in vitro, but how mTORC1 regulates this in vivo remains

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ebb96be3c1e410bb0994aa51d36226e9
https://pubmed.ncbi.nlm.nih.gov/35793635